Abstract
Accurately tracking a group of small biological organisms using algorithms to obtain their movement trajectories is essential to biomedical and pharmaceutical research. However, object mis-detection, segmentation errors and overlapped individual trajectories are particularly common issues that restrict the development of automatic multiple small organism tracking research. Extending on previous work, this paper presents an accurate and generalised Multiple Small Biological Organism Tracking System (MSBOTS), whose general feasibility is tested on three types of organisms. Evaluated on zebrafish, Artemia and Daphnia video datasets with a wide variety of imaging conditions, the proposed system exhibited decreased overall Multiple Object Tracking Precision (MOTP) errors of up to 77.59%. Moreover, MSBOTS obtained more reliable tracking trajectories with a decreased standard deviation of up to 47.68 pixels compared with the state-of-the-art idTracker system. This paper also presents a behaviour analysis module to study the locomotive characteristics of individual organisms from the obtained tracking trajectories. The developed MSBOTS with the locomotive analysis module and the tested video datasets are made freely available online for public research use.
Highlights
This paper extends on this existing work to present a novel behaviour analysis module to implement locomotive behaviour analysis such as estimating object velocities, accelerations and movement directions
To evaluate the performance of Multiple Small Biological Organism Tracking System (MSBOTS), microscopic videos of three types of small biological organisms were tested, where preliminary evaluation using zebrafish was presented in our previous work (Wang et al, 2018; Wang, 2018)
The detection and segmentation becomes more challenging when tracking small biological aquatic organisms compared with general objects, which in turn affects the subsequent organism tracking processes
Summary
Small biological organisms such as zebrafish larvae (genetically and physiologically similar to humans), Artemia franciscana, and Daphnia magna have become powerful models and are widely used to study human disease (James et al, 2019), pharmacology (Comeche et al, 2017) and ecotoxicology (James et al, 2019; Comeche et al, 2017; Poynton et al, 2007). MSBOTS: a multiple small biological organism tracking system robust against non-ideal detection and segmentation conditions.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
