MRNA Expression of Ovine Angiopoietin-like Protein 4 Gene in Adipose Tissues

Jing Zhang,Jian-Hua Liu,Wen-Zhong Liu,Li-Ying Qiao,Xia-Li Jia,Chen Liang,Jiong-Jie Jing

doi:10.5713/ajas.15.0090

Jing Zhang, Jian-Hua Liu + Show 5 more

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https://doi.org/10.5713/ajas.15.0090

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Abstract

Angiopoietin-like protein 4 (ANGPTL4) is involved in a variety of functions, including lipoprotein metabolism and angiogenesis. To reveal the role of ANGPTL4 in fat metabolism of sheep, ovine ANGPTL4 mRNA expression was analyzed in seven adipose tissues from two breeds with distinct tail types. Forty-eight animals with the gender ratio of 1:1 for both Guangling Large Tailed (GLT) and Small Tailed Han (STH) sheep were slaughtered at 2, 4, 6, 8, 10, and 12 months of age, respectively. Adipose tissues were collected from greater and lesser omental, subcutaneous, retroperitoneal, perirenal, mesenteric, and tail fats. Ontogenetic mRNA expression of ANGPTL4 in these adipose tissues from GTL and STH was studied by quantitative real time polymerase chain reaction. The results showed that ANGPTL4 mRNA expressed in all adipose tissues studied with the highest in subcutaneous and the lowest in mesenteric fat depots. Months of age, tissue and breed are the main factors that significantly influence the mRNA expression. These results provide new insights into ovine ANGPTL4 gene expression and clues for its function mechanism.

Highlights

ANGPTL4, known as a fasting-induced adipose factor, which is up-regulated by fasting, is one of the seven members of angiopoietin-like proteins (ANGPTLs)
Factors influencing ovine ANGPTL4 mRNA expression analysis of variance (ANOVA) results showed that breed (p
Abundance in Guangling Large Tailed (GLT) (4.566) was significantly higher than that in Small Tailed Han (STH) (1.753) suggesting that breed difference existed in the mRNA expression

Summary

Introduction

ANGPTL4, known as a fasting-induced adipose factor, which is up-regulated by fasting, is one of the seven members of angiopoietin-like proteins (ANGPTLs). It is involved in angiogenesis and encoded by ANGPTL4 gene. It is secreted by a variety of tissues, mainly by liver and white adipose tissues (WAT), and exists in either native (full-length) form with molecular mass of ~50 kDa (Mandard et al, 2006) or truncated forms both in human and mouse plasma (Mandard et al, 2004; Zhu et al, 2012). The native form was physically associated with high density lipoprotein, whereas truncated form was associated with low density lipoprotein.

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