Abstract
A new multi-quantum stimulated echo based pulsed gradient spin-echo sequence, MQ-PGSTE, has been developed for measuring translational diffusion. The new sequence provides a higher signal-to-noise ratio than the (Hahn spin-echo based) MAXY-D sequence at long diffusion times, and thus potentially affords better diffusion measurements on macromolecule samples. Based on multi-quantum coherence encoding, the MQ-PGSTE sequence needs considerably lower gradient strengths for diffusion characterization compared to standard single quantum pulsed gradient spin-echo sequences. By using the new sequence, the diffusion coefficient of l-[3- 13C]-alanine was found to be 8.1 ± 0.1 × 10 −10 m 2 s −1, which is in line with the value obtained by the use of the standard stimulated echo based pulsed gradient spin-echo sequence.
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