MP94-01 PROTECTIVE EFFECT OF PLATELET-RICH PLASMA ON URETHRAL STRICTURE MODEL OF MALE RATS

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology II1 Apr 2017MP94-01 PROTECTIVE EFFECT OF PLATELET-RICH PLASMA ON URETHRAL STRICTURE MODEL OF MALE RATS HASAN TAVUKCU, OMER AYTAÇ, FATIH ATUG, BURÇIN ALEV, OZGE CEVIK, AYSEN YARAT, SULE CETINEL, GÖKSEL SENER, and HALUK KULAKSIZOGLU HASAN TAVUKCUHASAN TAVUKCU More articles by this author , OMER AYTAÇOMER AYTAÇ More articles by this author , FATIH ATUGFATIH ATUG More articles by this author , BURÇIN ALEVBURÇIN ALEV More articles by this author , OZGE CEVIKOZGE CEVIK More articles by this author , AYSEN YARATAYSEN YARAT More articles by this author , SULE CETINELSULE CETINEL More articles by this author , GÖKSEL SENERGÖKSEL SENER More articles by this author , and HALUK KULAKSIZOGLUHALUK KULAKSIZOGLU More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.2904AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Urethral stricture formation is caused by fibrosis after excessive collagen formation to the any injury or trauma to the urethra. We evaluated the affects of platelet-rich plasma (PRP) on urethral stricture model of male rats. METHODS Urethral stricture model was performed by coagulation current to the male urethra. There were 4 groups of 6 rats in each one: control (C), C+PRP applied, urethral stricture (US), US+PRP applied. PRP was applied to the urethra after coagulation current induced injury as soon as possible. On 14th day all rats were sacrified and urethral tissues investigated for collagen type I (col I), collagen type III (col III), platelet-derived growth factor-A (PDGF-A), platelet-derived growth factor-B (PDGF-B) and transforming growth factor-B (TGF-B) with quantative real-time polymerase chain reaction (RT-PCR) and Western-Blot analysis. The efffect of urethral damage and healing was investigated for col I/col III ratio. RESULTS Collagen type I increase in fibrosis process in US is well defined. Collagen type I/ collagen type III ratio was significantly high in US group (*, p=0,000) than others while US+PRP group had comparable results with the control group (p=0,999). (Graphic 1) RT-PCR analysis of col I, col III, PDGF-A, PDGF-B and TGF-B was shown in table 1. Graphic 1: Col I/Col III type ratio of each group. Table 1: Results of RT-PCR analysis of col I, col III, PDGF-A, PDGF-B and TGF-B.(* Significant between US and US+PRP groups; & not significant between C and US+PRP groups; ** significant between C and US+PRP groups) CONCLUSIONS Our results show that PRP has a preventive effect on stricture formation in US rat model shown by its effect on collagen synthesis, especially in recurrent cases. Further studies that eventually show the effects of PRP on human tissues is necessary and promising. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e1245 Advertisement Copyright & Permissions© 2017MetricsAuthor Information HASAN TAVUKCU More articles by this author OMER AYTAÇ More articles by this author FATIH ATUG More articles by this author BURÇIN ALEV More articles by this author OZGE CEVIK More articles by this author AYSEN YARAT More articles by this author SULE CETINEL More articles by this author GÖKSEL SENER More articles by this author HALUK KULAKSIZOGLU More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...