MP91-08 TRANSCRIPTOME ANALYSIS OF TESTICULAR TISSUES IN MEN WITH NON-OBSTRUCTIVE AZOOSPERMIA WITH VARICOCELE AND PREDICTIVE FACTORS OF SPERM RECOVERY AFTER VARICOCELECTOMY

Abstract

You have accessJournal of UrologyInfertility: Epidemiology & Evaluation1 Apr 2016MP91-08 TRANSCRIPTOME ANALYSIS OF TESTICULAR TISSUES IN MEN WITH NON-OBSTRUCTIVE AZOOSPERMIA WITH VARICOCELE AND PREDICTIVE FACTORS OF SPERM RECOVERY AFTER VARICOCELECTOMY Koji Shiraishi, Shintaro Oka, and Hideyasu Matsuyama Koji ShiraishiKoji Shiraishi More articles by this author , Shintaro OkaShintaro Oka More articles by this author , and Hideyasu MatsuyamaHideyasu Matsuyama More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2595AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES This study evaluated the ability of testicular histopathology to predict the success of microsurgical varicocelectomy (Vx) in patients with non-obstructive azoospermia (NOA). We used next-generation sequencing to compare the transcriptomes of Vx-responsive and non-responsive testes to identify the factors that predict sperm recovery in the ejaculate. METHODS Eighty-three men with NOA and a left varicocele (grade 2 or 3) underwent microsurgical Vx with simultaneous testicular biopsy for diagnostic and therapeutic purposes under the IRB approval and written consent. The testicular histopathology was categorized as Sertoli cell only (SCO), maturation arrest (MA), and hypospermatogenesis (Hypo). The transcriptome results from the Illumina platform were expressed as fragments per kilobase and a Cuff Diff analysis was performed to compare individual differences. The data were compared with those of testicular tissues from men with obstructive azoospermia (OA). The outcomes of sperm recovery were investigated with respect to patient age, testicular volume, varicocele grade, FSH, and testicular histology. RESULTS The mean age and FSH were 34 years and 12.3 IU/L, respectively. There was sperm recovery in 20 patients (24%) within 12 months after Vx. There was recovery in all types of patients; SCO: 1/43 (2%), MA: 10/27 (37%), and Hypo: 9/13 (69%). The transcriptome analysis of NOA samples revealed that 261 genes were upregulated and 580 genes were downregulated with a cutoff of 2-fold change compared to OA samples. The genes were clustered mainly into the following categories: cell cycle regulation, transcription/translation, cell differentiation, and mitochondrial dysfunction/oxidative stress. The transcription of the cell cycle regulation gene cluster and immunohistological expression of proliferating cell nuclear antigen (PCNA) were significantly higher in Vx-responsive men (n=10) than in Vx non-responsive men (n=17) with MA. The multivariate analysis showed that Hypo and PCNA expression predict sperm recovery. CONCLUSIONS Hypo patients identified by testicular histology have an increased frequency of sperm recovery than SCO patients after Vx. The transcriptome analysis of MA patients revealed a distinct difference in the transcription of cell cycle regulation genes between Vx-responsive and non-responsive patients. Cumulatively, our findings indicate that cell cycle assessment can predict sperm recovery and could improve our understanding of varicocele pathophysiology. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1156 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Koji Shiraishi More articles by this author Shintaro Oka More articles by this author Hideyasu Matsuyama More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...