MP88-12 HIGHLY SENSITIVE AND SPECIFIC NOVEL BIOMARKERS FOR THE DIAGNOSIS OF TRANSITIONAL BLADDER CARCINOMA

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology V1 Apr 2016MP88-12 HIGHLY SENSITIVE AND SPECIFIC NOVEL BIOMARKERS FOR THE DIAGNOSIS OF TRANSITIONAL BLADDER CARCINOMA Hong Koo Ha, Prashant Kumar, Sayantani Nandi, Tuan Zea Tan, Siok Ghee Ler, Kee Seng Chia, Wei-Yen Lim, Ja Yoon Ku, Chan Ho Lee, Kyung Lee, Kyoung Hwan Kim, Seung Ryong Baek, Hyo Jung Park, Jeong Zoo Lee, and Jean Paul Thiery Hong Koo HaHong Koo Ha More articles by this author , Prashant KumarPrashant Kumar More articles by this author , Sayantani NandiSayantani Nandi More articles by this author , Tuan Zea TanTuan Zea Tan More articles by this author , Siok Ghee LerSiok Ghee Ler More articles by this author , Kee Seng ChiaKee Seng Chia More articles by this author , Wei-Yen LimWei-Yen Lim More articles by this author , Ja Yoon KuJa Yoon Ku More articles by this author , Chan Ho LeeChan Ho Lee More articles by this author , Kyung LeeKyung Lee More articles by this author , Kyoung Hwan KimKyoung Hwan Kim More articles by this author , Seung Ryong BaekSeung Ryong Baek More articles by this author , Hyo Jung ParkHyo Jung Park More articles by this author , Jeong Zoo LeeJeong Zoo Lee More articles by this author , and Jean Paul ThieryJean Paul Thiery More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2430AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Transitional bladder carcinoma (BCa) is prevalent in developed countries, particularly among men. Given that these tumors frequently recur or progress, the early detection and subsequent monitoring of BCa at different stages is critical. Current BCa diagnostic biomarkers are not sufficiently sensitive for substituting or complementing invasive cystoscopy. Here, we sought to identify a robust set of urine biomarkers for BCa detection. METHODS Using a high-resolution, mass spectrometry-based, quantitative proteomics approach, we measured, compared and validated protein variations in 451 voided urine samples from healthy subjects, non-bladder cancer patients and patients with non-invasive and invasive BCa. We identified five robust biomarkers: Coronin-1A, Apolipoprotein A4, Semenogelin-2, Gamma synuclein and DJ-1/PARK7. In diagnosing Ta/T1 BCa, these biomarkers achieved an AUC of 0.92 and 0.98, respectively, using ELISA and western blot data (sensitivity, 79.2% and 93.9%; specificity, 100% and 96.7%, respectively). In diagnosing T2/T3 BCa, an AUC of 0.94 and 1.0 was attained (sensitivity, 86.4% and 100%; specificity, 100%) using the same methods. RESULTS Urine samples from 66 healthy subjects, 110 Ta/T1 BCa patients and 63 T2/T3 BCa patients were analyzed using western blotting. All BCa urine samples exhibited an elevated level in at least three out of the five biomarkers. ROC curve, established using band intensity values, showed an AUC of 0.98 and 1.0 for healthy versus Ta/T1 and healthy versus T2/T3, respectively, using the five biomarkers in combination CONCLUSIONS We established a robust, urinary protein biomarker panel for BCa diagnosis. This panel consists of five biomarkers with significantly higher sensitivity and specificity than that of the currently available, noninvasive diagnostic assays for the detection of BCa. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1133 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Hong Koo Ha More articles by this author Prashant Kumar More articles by this author Sayantani Nandi More articles by this author Tuan Zea Tan More articles by this author Siok Ghee Ler More articles by this author Kee Seng Chia More articles by this author Wei-Yen Lim More articles by this author Ja Yoon Ku More articles by this author Chan Ho Lee More articles by this author Kyung Lee More articles by this author Kyoung Hwan Kim More articles by this author Seung Ryong Baek More articles by this author Hyo Jung Park More articles by this author Jeong Zoo Lee More articles by this author Jean Paul Thiery More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...