MP82-05 FRAGMENT LENGTH AND CONCENTRATION OF PLASMA CFDNA ARE POTENTIAL BIOMARKERS FOR UPPER TRACT UROTHELIAL CARCINOMA DIAGNOSIS

Abstract

You have accessJournal of UrologyBladder Cancer: Upper Tract Transitional Cell Carcinoma II (MP82)1 Apr 2020MP82-05 FRAGMENT LENGTH AND CONCENTRATION OF PLASMA CFDNA ARE POTENTIAL BIOMARKERS FOR UPPER TRACT UROTHELIAL CARCINOMA DIAGNOSIS Kosuke Nakano*, Motohide Uemura, Eisuke Tomiyama, Yoko Koh, Makoto Matsushita, Yujiro Hayashi, Yu Ishizuya, Cong Wang, Taigo Kato, Koji Hatano, Atsunari Kawashima, Takeshi Ujike, Kazutoshi Fujita, and Norio Nonomura Kosuke Nakano*Kosuke Nakano* More articles by this author , Motohide UemuraMotohide Uemura More articles by this author , Eisuke TomiyamaEisuke Tomiyama More articles by this author , Yoko KohYoko Koh More articles by this author , Makoto MatsushitaMakoto Matsushita More articles by this author , Yujiro HayashiYujiro Hayashi More articles by this author , Yu IshizuyaYu Ishizuya More articles by this author , Cong WangCong Wang More articles by this author , Taigo KatoTaigo Kato More articles by this author , Koji HatanoKoji Hatano More articles by this author , Atsunari KawashimaAtsunari Kawashima More articles by this author , Takeshi UjikeTakeshi Ujike More articles by this author , Kazutoshi FujitaKazutoshi Fujita More articles by this author , and Norio NonomuraNorio Nonomura More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000974.05AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Upper tract urothelial carcinoma (UTUC) is diagnosed by urine cytology and imaging tests such as computed tomography. Reliable blood biomarkers have yet to be found. Circulating cell-free DNA (cfDNA) is an emerging resource for the diagnosis and prognosis of various cancers. This study aims to identify novel blood biomarkers from plasma cfDNA for UTUC. METHODS: Plasma cfDNA was extracted from untreated UTUC patients (n=47) and healthy controls (n=26). The fragment length of cfDNA was measured using a microfluidics-based platform (Agilent 2100 Bioanalyzer®) and the concentration of cfDNA was determined using quantitative real-time PCR of ACTB as the target gene. Fragment length and concentration of cfDNA were compared in healthy controls and UTUC groups, and the diagnostic ability of UTUC was evaluated by receiver operating characteristic (ROC) curve analysis. RESULTS: The median ages of the healthy controls and UTUC groups were 68 (50 - 77) and 73 (51 - 88) years, respectively. In 45 patients with UTUC, the clinical stage distribution (stage Ⅰ/Ⅱ/Ⅲ/Ⅳ) was 14 (29.8%), 19 (40.4%), 6 (12.8), 8 (17.0%), respectively. The median fragment length of cfDNA in UTUC patients was significantly shorter than that in healthy controls (166 vs. 168.5 bp, p=0.008). The median concentration of cfDNA from UTUC patients was significantly higher than that from healthy controls (5081 vs. 3780 copies / ml, p=0.040). ROC curve analysis revealed that fragment length of cfDNA had sensitivity of 57% and specificity of 73% (AUC 0.69), and concentration of cfDNA had sensitivity of 33% and specificity of 95% (AUC 0.66) for the presence of UTUC. Furthermore, the median fragment length of cfDNA in stage Ⅲ and Ⅳ patients was shorter than that in stage Ⅰand Ⅱ patients (160 vs. 167 bp, p=0.031), and the median concentration of cfDNA from stage Ⅲ and Ⅳ patients was higher than that from stage Ⅰand Ⅱ patients (11153 vs. 4273 copies/ml, p=0.040). CONCLUSIONS: Fragment length and concentration of cfDNA are potential markers for the diagnosis of UTUC. Source of Funding: This work was supported by a KAKENHI grant (19K18558). © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e1247-e1247 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kosuke Nakano* More articles by this author Motohide Uemura More articles by this author Eisuke Tomiyama More articles by this author Yoko Koh More articles by this author Makoto Matsushita More articles by this author Yujiro Hayashi More articles by this author Yu Ishizuya More articles by this author Cong Wang More articles by this author Taigo Kato More articles by this author Koji Hatano More articles by this author Atsunari Kawashima More articles by this author Takeshi Ujike More articles by this author Kazutoshi Fujita More articles by this author Norio Nonomura More articles by this author Expand All Advertisement PDF downloadLoading ...