MP81-19 EFFECTS OF MESENCHYMAL STEM CELLS ON ERECTILE FUNCTION IN A PELVIC NEUROVASCULAR TRAUMA MODEL

Abstract

You have accessJournal of UrologyStem Cell Research: Stem Cell Research II1 Apr 2018MP81-19 EFFECTS OF MESENCHYMAL STEM CELLS ON ERECTILE FUNCTION IN A PELVIC NEUROVASCULAR TRAUMA MODEL Alexandria Hertz, Evalyn George, Amanda Reed-Maldonado, Timothy Brand, and Shashikumar Salgar Alexandria HertzAlexandria Hertz More articles by this author , Evalyn GeorgeEvalyn George More articles by this author , Amanda Reed-MaldonadoAmanda Reed-Maldonado More articles by this author , Timothy BrandTimothy Brand More articles by this author , and Shashikumar SalgarShashikumar Salgar More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2727AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Erectile dysfunction (ED) following trauma is challenging as traditional therapies are often ineffective. Low-energy shockwave therapy (LESWT) has shown promise in recovery of erectile function. This study aimed to determine whether the combination of mesenchymal stem cells (MSC), LESWT, and phosphodiesterase 5-inhibitors (PDE5i) would result in improved erectile function. METHODS Thirty-six Lewis rats, aged 10-12 weeks, were divided into five groups: sham operation, bilateral cavernous nerve crush injury and bilateral internal pudendal bundle ligation without treatment (control), injury with MSC injection, injury with LESWT and MSC, injury with MSC and PDE5i, injury with LESWT, PDE5i, and MSC (n=6 rats/group). The groups receiving MSCs were given a one-time intracavernosal injection of bone marrow-derived MSCs (2 million in 100µl of normal saline) at the time of injury. Forty-eight hours after injury groups were started on three times weekly LESWT (1000 pulses at 0.06 mJ/mm2 and 3Hz) or sildenafil was given by mouth mixed into daily drinking water at 5-20 mg/kg/day (water intake ad libitum) with a 1% sucrose additive, for four weeks. Following one week washout period (week 5), intracavernous pressure (ICP) was assessed at week 6 as a final outcome measure. RESULTS Pelvic neurovascular injury resulted in a significant decrease in erectile function as measured by maximum ICP, average ICP and change in ICP (p=0.003, 0.001, 0.007, respectively). MSC therapy alone resulted in partial recovery (average ICP, change in ICP compared to control group, p=0.05, 0.037). When compared to the sham group, combination LESWT and MSC resulted in partial recovery (p=0.08). The other combination therapies tested did not show significant (p>0.05) erectile recovery. CONCLUSIONS MSC alone and in combination with LESWT produced partial improvement of erectile function in a rat model of pelvic nerve injury. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e1104 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Alexandria Hertz More articles by this author Evalyn George More articles by this author Amanda Reed-Maldonado More articles by this author Timothy Brand More articles by this author Shashikumar Salgar More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...