MP81-18 INTRACAVERNOUS INJECTION OF BONE MARROW MESENCHYMAL STEM CELLS MODIFIED WITH PDE5-RNAI IMPROVES ERECTILE DYSFUNCTION IN AGED RATS

Abstract

You have accessJournal of UrologyStem Cell Research: Stem Cell Research II1 Apr 2018MP81-18 INTRACAVERNOUS INJECTION OF BONE MARROW MESENCHYMAL STEM CELLS MODIFIED WITH PDE5-RNAI IMPROVES ERECTILE DYSFUNCTION IN AGED RATS Hengjun Xiao, Weixin Yan, Yubin Cui, Jun Chen, and Liaoyuan Li Hengjun XiaoHengjun Xiao More articles by this author , Weixin YanWeixin Yan More articles by this author , Yubin CuiYubin Cui More articles by this author , Jun ChenJun Chen More articles by this author , and Liaoyuan LiLiaoyuan Li More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2726AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To determine the effect of intracavernous injection of bone marrow mesenchymal stem cells (BMSCs) genetically modified with PDE5-RNAi on erectile dysfunction (ED) in aged rats. METHODS BMSCs collected from Sprague-Dawley (SD) male rats of six weeks were transfected with lentivirus modified with PDE5-RNAi. Sixty male SD rats were equally divided into six groups: (i) A control group of normal rats (n=10), (ii) A control group of Aged rats (n=10), (iii) A negative control group of aged rats treated with PBS (n=10), (iv) aged rats treated with BMSCs alone (n=10), (v) aged rats treated with BMSCs modified with lentivirus-PDE5-RNAi (n=10), (vi) aged rats treated with lentivirus-PDE5-RNAi (n=10). ED was screened with apomorphine (100 mg/kg). Cell injections occurred directly into the corpora cavernosa (1×106 cells). The ratio of intracavernosal pressure (ICP) to mean arterial pressure (MAP), expression of endothelial markers (such as CD31 and eNOS), smooth muscle markers (such as a-SMA and desmin), VEGF, b-FGF, Angiopoietin-1 and the relative area of smooth muscle to collagen using masson trichrome staining were assessed for each group. RESULTS Implanted BMSCs modified with PDE5-RNAi displayed a significantly raised ICP and ICP/MAP ratio (p < 0.05) compared with BMSCs and lentivirus-PDE5-RNAi alone in 14 days after intracavernous injection. Immunofluorescence staining displayed expression of CD31, eNOS and a-SMA was increased in BMSCs alone or BMSCs modified with PDE5-RNAi groups in aged rats after 2 weeks. Western blot analysis and quantitative analysis for western blot confirmed the significantly increased expression of CD31, eNOS and a-SMA (p < 0.05).VEGF, b-FGF, and Angiopoietin-1 were also significantly elevated 2 weeks following BMSCs modified with PDE5-RNAi injection (p < 0.05). After receiving intracaverous injection of BMSCs modified with PDE5-RNAi, smooth muscle cells/collagen ratio significantly increased confirmed by Masson's trichrome-staining. CONCLUSIONS These results suggest that BMSCs modified with PDE5-RNAi treatment can enhance the recovery of erectile function in aged rats. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e1103-e1104 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Hengjun Xiao More articles by this author Weixin Yan More articles by this author Yubin Cui More articles by this author Jun Chen More articles by this author Liaoyuan Li More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...