MP81-02 CONTROLLED DELIVERY OF STEM CELL-DERIVED TROPHIC FACTORS TO TREAT KIDNEY INJURY

Abstract

You have accessJournal of UrologyStem Cell Research: Stem Cell Research II1 Apr 2018MP81-02 CONTROLLED DELIVERY OF STEM CELL-DERIVED TROPHIC FACTORS TO TREAT KIDNEY INJURY Doosang Kim, Hyungeun Yim, Hyun Chul Chung, Brian Shing, Kyung Hyun Moon, Sunil George, John Jackson, In Kap Ko, James Yoo, and Anthony Atala Doosang KimDoosang Kim More articles by this author , Hyungeun YimHyungeun Yim More articles by this author , Hyun Chul ChungHyun Chul Chung More articles by this author , Brian ShingBrian Shing More articles by this author , Kyung Hyun MoonKyung Hyun Moon More articles by this author , Sunil GeorgeSunil George More articles by this author , John JacksonJohn Jackson More articles by this author , In Kap KoIn Kap Ko More articles by this author , James YooJames Yoo More articles by this author , and Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2710AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Renal disease is a worldwide health issue. Besides transplantation, current therapies revolve around dialysis, which is limited to delaying disease progression through filtering metabolic wastes in blood. However, dialysis is unable to replace other renal functions, such as synthesizing erythropoietin. To address these limitations, cell-based approaches have been proposed to restore damaged kidneys as an alternative to current therapies. Particularly, recent studies have shown that stem cell-derived secretomes could enhance tissue regeneration. This study explored a gel-based delivery system for controlled delivery of trophic factors secreted from human placental stem cells (hPSC) (conditioned medium: CM) and evaluated the effect of trophic factors on renal regeneration in vitro and in vivo. METHODS hPSC were grown under normal culture environment, and conditioned media were collected and concentrated. The concentrated conditioned media containing trophic factors were combined with Platelet-rich plasma (PRP), which served as a delivery vehicle. To test the feasibility of controlled delivery, CM was encapsulated within the PRP, followed by assessing the release kinetics of CM from the gel. The gel formulation was injected into a rat acute kidney injury model for analysis. RESULTS In vitro cell viability and proliferation assays demonstrated that CM treatment significantly enhanced cell proliferation when compared with the control without CM. The release profiles show that CM can be released from PRP in a controlled manner by altering gel stiffness. An In vivo study using a rat acute kidney injury model showed that CM delivery using the gel system into the injured kidney tissue facilitated less renal tissue damage, leading to rapid functional recovery than that of saline, CM or vehicle only group. CONCLUSIONS These results suggest that the delivery of hPSC-derived trophic factors in a controlled manner may contribute to effective kidney repair from renal tissue injury. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e1097 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Doosang Kim More articles by this author Hyungeun Yim More articles by this author Hyun Chul Chung More articles by this author Brian Shing More articles by this author Kyung Hyun Moon More articles by this author Sunil George More articles by this author John Jackson More articles by this author In Kap Ko More articles by this author James Yoo More articles by this author Anthony Atala More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...