MP81-01 A NOVEL ROLE OF EPITHELIAL PERMEABILITY IN VISCERAL ORGAN CROSS TALK

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Female Urology1 Apr 2015MP81-01 A NOVEL ROLE OF EPITHELIAL PERMEABILITY IN VISCERAL ORGAN CROSS TALK Alexander Parker, Ehsan Mohommadi, Karl Tyler, Robert E. Hurst, and Beverley Greenwood-Van Meerveld Alexander ParkerAlexander Parker More articles by this author , Ehsan MohommadiEhsan Mohommadi More articles by this author , Karl TylerKarl Tyler More articles by this author , Robert E. HurstRobert E. Hurst More articles by this author , and Beverley Greenwood-Van MeerveldBeverley Greenwood-Van Meerveld More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2879AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Painful bladder syndrome (PBS) and irritable bowel syndrome (IBS) are often comorbid conditions thought to be due to visceral organ cross communication. It is our overall hypothesis that changes in epithelial permeability may represent a novel mechanism for visceral organ crosstalk. In support, we previously found that following acute bladder damage, there was a marked increase in the permeability of both the bladder and the undamaged colon. However, it is currently unknown whether damage to the colon will alter permeability in the undamaged bladder. The goal of the current study was to test the hypothesis that an acute colitis increases permeability in the undamaged bladder. METHODS Experiments were performed in fasted, ovariectomized (OVX) female Sprague Dawley rats (n=24). An acute colitis was produced by an enema of trinitrobenzene sulfonic acid (TNBS) (50 mg/kg) with saline-enema treated rats serving as controls. The severity of colonic damage was assessed using an in vivo disease activity index (DAI) score and via post mortem histology. Colon and bladder tissue were harvested after 1 day or 3 days. Bladder and colonic tissue was mounted into modified Ussing chambers and bathed in Kreb's solution at 37°C. After a stabilization period, permeability was assessed electrophysiologically via transepithelial resistance (TEER) and via the flux of a macromolecular marker (fluorescein isothiocynate-dextrin [FITC-4 KD). RESULTS Compared to saline-treated controls, TNBS-treated rats showed a significantly (P<0.05) higher DAI on days 1 and 3. Exposing the colon to TNBS induced marked colonic damage, but no bladder damage as defined histologically. We found that in response to acute colonic damage, bladder TEER significantly decreased after 1 day (P<0.0001) and after 3 days (P<0.0001), and bladder macromolecular permeability significantly increased after 1 day (P<0.05) and 3 days (P<0.01) when compared to saline-treated controls. In the colon from TNBS-treated rats there was a significant decrease in TEER, and a significant increase in macromolecular transport of FITC-dextrin after 1 day (P<0.005) and 3 days (P<0.0001) compared to saline-treated controls. CONCLUSIONS These data demonstrate that following acute colon damage there was a marked increase in the permeability of the bladder in the absence of any histological damage to the bladder. This new data reinforces the notion that changes in epithelial permeability may represent a novel mechanism for visceral organ crosstalk and may be important in the overlapping symptomology of PBS and IBS. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e1027-e1028 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Alexander Parker More articles by this author Ehsan Mohommadi More articles by this author Karl Tyler More articles by this author Robert E. Hurst More articles by this author Beverley Greenwood-Van Meerveld More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...