MP8-04 UNDERACTIVE BLADDER IN OBESE-PRONE RATS FED A HIGH FAT DIET

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research I1 Apr 2015MP8-04 UNDERACTIVE BLADDER IN OBESE-PRONE RATS FED A HIGH FAT DIET Nazema Siddiqui, Alexis Dieter, Cindy Amundsen, Jillene Brooks, Danielle Degoski, and Matthew Fraser Nazema SiddiquiNazema Siddiqui More articles by this author , Alexis DieterAlexis Dieter More articles by this author , Cindy AmundsenCindy Amundsen More articles by this author , Jillene BrooksJillene Brooks More articles by this author , Danielle DegoskiDanielle Degoski More articles by this author , and Matthew FraserMatthew Fraser More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.271AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Changes in systemic metabolism lead to alterations in lower urinary tract (LUT) physiology. High fat diets (HFD) are implicated in the development of insulin resistance. Obese Prone (OP) and Obese Resistant (OR) rats (Charles River) are used in obesity research, as OP rats become obese on HFD while OR remain lean. To assess the effect of obesity on LUT function, we characterized LUT function in both rat strains during chronic HFD feeding. METHODS Four OP and 4 OR female rats were placed on the same HFD at 9 weeks (wks) of age (30% fat for 12 wk, followed by 60% fat for 4 wk, Research Diets). Conscious restrained cystometry was performed at 7, 11 and 15 wk HFD. Body mass index (BMI) was calculated, and serum glucose was measured. For all cystometric evaluations, single fill cystometrograms were performed to determine true bladder capacity (TBC), followed by 60 min of continuous cystometry to determine functional bladder capacity (FBC). Voiding efficiency (%VE) was calculated as follows: %VE = [average FBC/TBC]∗100. For animals showing overflow incontinence (OI), TBC was a priori considered to be 5 milliliters. RESULTS Mean BMIs were higher in OP compared to OR rats (0.64 vs. 0.46 at 7wk, 0.72 vs. 0.52 at 11 wk, 0.74 vs. 0.52 at 15 wk, respectively; p = 0.002). At 15 wk HFD, there were no significant differences in mean blood glucose between OP and OR groups (102 +/-14 vs. 97 +/- 11, respectively; p=0.58). TBC and %VE were compared across all time points and results are shown in the figure. At 7 wk HFD, all rats exhibited normal voiding patterns. At 11 wk HFD, 2/4 (50%) of OP rats exhibited OI while the remaining 2/4 OP and all 4 OR rats exhibited normal voiding patterns. At 15 wk HFD, one of the OP rats with OI at 11 wk HFD could no longer be assessed due to catheter issues. Of the remaining OP rats, 2/3 (66%) exhibited overflow incontinence while all OR rats showed normal voiding patterns. Mean %VE decreased in the OP group. CONCLUSIONS Obese non-diabetic animals exhibited elevated bladder capacities, to the point of OI in the majority, and decreased voiding efficiency after 15 wk HFD. OP rats given a chronic HFD may serve as a promising new model to study underactive bladder. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e74 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Nazema Siddiqui More articles by this author Alexis Dieter More articles by this author Cindy Amundsen More articles by this author Jillene Brooks More articles by this author Danielle Degoski More articles by this author Matthew Fraser More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...