MP79-01 VITRIFICATION OF METANEPHROS: WOULD IT BE THE FUTURE NEPHRO-BIOBANK TO PROVIDE AN UNLIMITED SUPPLY OF KIDNEYS FOR THE HUMAN BEING?

Abstract

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation & Vascular Surgery I1 Apr 2015MP79-01 VITRIFICATION OF METANEPHROS: WOULD IT BE THE FUTURE NEPHRO-BIOBANK TO PROVIDE AN UNLIMITED SUPPLY OF KIDNEYS FOR THE HUMAN BEING? Cesar Vera-Donoso, Joaquín García-Domínguez, Silvia Vicente-Ferrer, Estrella Jimenez-Trigos, José Vicente-Antón, and Francisco Marco-Jiménez Cesar Vera-DonosoCesar Vera-Donoso More articles by this author , Joaquín García-DomínguezJoaquín García-Domínguez More articles by this author , Silvia Vicente-FerrerSilvia Vicente-Ferrer More articles by this author , Estrella Jimenez-TrigosEstrella Jimenez-Trigos More articles by this author , José Vicente-AntónJosé Vicente-Antón More articles by this author , and Francisco Marco-JiménezFrancisco Marco-Jiménez More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2858AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Several adverse are factors that require ensuring safe and unlimited supply of kidneys. It means a nephro-biobank. Last years, xeno-metanephroi transplantation in adult hosts showed that theses growing kidneys underwent maturation, exhibiting functional properties, and avoiding hyperacute and acute humoral rejection post-transplantation in non-immunosuppressed hosts. In any event, xenotransplants or lab-grown organs from regenerative medicine, organ cryopreservation will be essential because we need to guarantee the ability to physically distribute the organs to patients in need and to produce them in a way that allows adequate inventory control and quality assurance. Our goal was to know if it is possible to store these metanephroi preserving their viability for transplantation. METHODS Metanephroi from fifteen-days old of rabbit embryos were carefully microdissected and vitrified. Following the minimum essential volume method and using Cryotop as a device and M22 as vitrification solution we performed vitrification of metanephroi. After three months of storage in liquid nitrogen, metanephroi were warmed and transplanted by minimally invasive laparoscopy surgery into 5-month-old immune-competent New Zealand rabbits. Hosts received metanephroi into the retroperitoneal fat, near to the renal vessels. Each host received 4 metanephroi. We compared this group (A) with other that underwent fresh metanephroi transplantation (B). A total of 19 vitrified metanephroi and 20 fresh metanephoi were transplanted RESULTS Significant growth and full differentiation into mature glomeruli and tubuli was observed in all the new kidneys recovered 21 days after transplantation when host were sacrificed. In group A, 6 metanephroi (31.6%) were successfully grown after vitrification. Similarly, 7 metanephroi (43.7%) were successfully grown in fresh group. Other authors have already shown that transplants of kidney precursors are able to filter blood, produce urine and accomplish metabolic functions (renin and erythropoietin production) CONCLUSIONS These encouraging results, for the first time reported in the medical and research literature, imply that metanephoi not only survived vitrification, but they vascularized and developed morphologically normal glomeruli after their allotransplantation without immune-supression and that it is possible to create a long-term nephro-biobank. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e1010 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Cesar Vera-Donoso More articles by this author Joaquín García-Domínguez More articles by this author Silvia Vicente-Ferrer More articles by this author Estrella Jimenez-Trigos More articles by this author José Vicente-Antón More articles by this author Francisco Marco-Jiménez More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...