MP78-06 THE EFFECT OF CIRCULATING TUMOR CELLS AND COPY NUMBER VARIATIONS OF CIRCULATING TUMOR DNA ON SURVIVAL IN BLADDER CANCER PATIENTS TREATED WITH RADICAL CYSTECTOMY

Abstract

You have accessJournal of UrologyBladder Cancer: Invasive VI1 Apr 2018MP78-06 THE EFFECT OF CIRCULATING TUMOR CELLS AND COPY NUMBER VARIATIONS OF CIRCULATING TUMOR DNA ON SURVIVAL IN BLADDER CANCER PATIENTS TREATED WITH RADICAL CYSTECTOMY Armin Soave, Michael Rink, Heidi Schwarzenbach, Malte Vetterlein, Jessica Rührup, Oliver Engel, Roland Dahlem, Margit Fisch, Klaus Pantel, and Sabine Riethdorf Armin SoaveArmin Soave More articles by this author , Michael RinkMichael Rink More articles by this author , Heidi SchwarzenbachHeidi Schwarzenbach More articles by this author , Malte VetterleinMalte Vetterlein More articles by this author , Jessica RührupJessica Rührup More articles by this author , Oliver EngelOliver Engel More articles by this author , Roland DahlemRoland Dahlem More articles by this author , Margit FischMargit Fisch More articles by this author , Klaus PantelKlaus Pantel More articles by this author , and Sabine RiethdorfSabine Riethdorf More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2554AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To investigate detection and oncological impact of circulating tumor cells (CTC) in bladder cancer patients with presence of copy number variations (CNV) of circulating, cell-free DNA (cfDNA) treated with radical cystectomy (RC). METHODS The study included 85 bladder cancer patients, who were prospectively enrolled and treated with RC without neoadjuvant chemotherapy at our institution between 2011 and 2014. Blood samples were obtained from all patients preoperatively. For CTC analysis, 7.5ml blood was collected in CellSave tubes and analyzed with the standardized CellSearch® system (Janssen). Serum was prepared from 6ml whole blood, and the PME DNA Extraction kit (Analytik Jena) was used to extract circulating DNA. Multiplex ligation-dependent probe amplification (MLPA) was used to characterize CNV of cfDNA. In a single reaction, MLPA allows analyzing CNV in 43 chromosomal regions containing 37 genes. RESULTS MLPA was suitable for the characterization of CNV in 72 patients (84.7%). Data on CTC was available in 45 of these patients (62.5%). In total, 7 patients (15.6%) had presence of CTC with a median CTC count of one (interquartile range (IQR): 1-3). In 21 patients (46.7%), one to 6 deleted and/or amplified chromosomal regions were detected with a median CNV count of 2 (IQR: 1-2). Overall, most changes were located in the genes CDH1, RIPK2 and ZFHX3 in 8 patients (17.8%), 6 patients (13.3%) and 5 patients (11.1%). Chromosomal aberrations were found most frequently on chromosome 8 in 8 patients (17.8%). Overall, presence of CTC was not associated with CNV status. However, presence of CTC was associated with copy number losses in miR-15a (p=0.011). Patients with presence of CTC had reduced recurrence-free survival compared to patients without CTC (p=0.012), In combined Kaplan-Meier analysis, patients with presence of CTC plus presence of CNV had reduced cancer-specific and recurrence-free survival compared to patients without CTC but with presence of CNV (p≤0.035). In addition, patients with presence of CTC plus presence of CNV had reduced recurrence-free survival compared to patients without presence of CTC and without presence of CNV (p=0.028). CONCLUSIONS CTC are detectable in bladder cancer patients with CNV of various genes. The presence of CTC seems to be associated with CNV of specific genes. CTC have a negative impact on survival in patients with and without CNV of cfDNA. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e1039 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Armin Soave More articles by this author Michael Rink More articles by this author Heidi Schwarzenbach More articles by this author Malte Vetterlein More articles by this author Jessica Rührup More articles by this author Oliver Engel More articles by this author Roland Dahlem More articles by this author Margit Fisch More articles by this author Klaus Pantel More articles by this author Sabine Riethdorf More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...