MP78-06 COLLAGEN IS MORE ABUNDANT AND STRUCTURALLY ALTERED IN LICHEN SCLEROSUS

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP78-06 COLLAGEN IS MORE ABUNDANT AND STRUCTURALLY ALTERED IN LICHEN SCLEROSUS Ethan Richmond, Melissa Champer, Eduardo Mora, Wei Huang, Paul Campagnola, and Matthew D Grimes Ethan RichmondEthan Richmond More articles by this author , Melissa ChamperMelissa Champer More articles by this author , Eduardo MoraEduardo Mora More articles by this author , Wei HuangWei Huang More articles by this author , Paul CampagnolaPaul Campagnola More articles by this author , and Matthew D GrimesMatthew D Grimes More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003355.06AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Lichen sclerosus (LS) is a chronic inflammatory disease of the genital epithelium that results in fibrosis and severe urethral stricture disease in affected men. Altered physical properties of fibrotic tissue may result from increased collagen content (generally from increased production) and/or from altered collagen structure (generally from fiber cross-linking) and are undefined in LS. We aim to test the hypothesis that genital skin and male urethra affected by LS has increased collagen content and altered collagen structure. METHODS: Collagen was stained with picrosirius red in human urethral, vulvar, and foreskin specimens with and without LS. An observer blinded to LS status and tissue type acquired three 20x stromal images from each specimen. Using Image J software, we quantified and compared 1) collagen content (using two metrics: collagen proportionate area [CPA] and collagen fiber count), 2) collagen fiber length and width, and 3) collagen structure using the texture analysis technique gray level co-localization matrix (GLCM) with respect to LS status and tissue type. Pairwise comparisons were made using the Student’s T test with respect to LS status and tissue type. RESULTS: We analyzed 23 LS specimens (vulva n=9, urethra n=7, foreskin n=7) and 29 non-LS specimens (vulva n=9, urethra n=7, foreskin n=13). Fiber count and CPA were significantly higher in all LS specimens compared to non-LS specimens: CPA mean (SD): LS 0.971 (0.03) vs no-LS 0.948 (0.02), p<0.007; fiber count mean (SD): LS 2906 (127) vs no-LS 2509 (78) fibers; p=0.003 (Figure 1). Collagen fiber width and length were similar with respect to LS status. GLCM analysis showed decreased inverse difference moment and increased entropy in LS tissues indicative of less homogeneous and more disorganized tissue structure (p<0.001) (Figure 1). CONCLUSIONS: LS tissues have increased collagen content compared to non-LS tissues. Quantitative assessment of collagen organization, using GLCM, revealed less homogeneity and more disorganization of collagen in LS compared to non-LS tissues. Taken together, our findings suggest that alterations in physical tissue properties seen in LS may be due to both increased collagen abundance and altered structure. Source of Funding: K12DK100022NIH/NIDDK © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e1133 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ethan Richmond More articles by this author Melissa Champer More articles by this author Eduardo Mora More articles by this author Wei Huang More articles by this author Paul Campagnola More articles by this author Matthew D Grimes More articles by this author Expand All Advertisement PDF downloadLoading ...