MP76-08 THE EFFECT OF BOSENTAN, AN ENDOTHELIN-1 RECEPTOR ANTAGONIST ON TESTICULAR TISSUE OXIDANT AND ANTIOXIDANT LEVELS IN DIABETIC RATS

Abstract

You have accessJournal of UrologyInfertility: Basic Research, Physiology, Pathophysiology1 Apr 2015MP76-08 THE EFFECT OF BOSENTAN, AN ENDOTHELIN-1 RECEPTOR ANTAGONIST ON TESTICULAR TISSUE OXIDANT AND ANTIOXIDANT LEVELS IN DIABETIC RATS Erdal Alkan, RüstemAnýl Urgan, Mehmet Murad Basar, Zekai Halýcý, Mevlana Derya Balbay, Harun Un, and Hasan Tarik Atmaca Erdal AlkanErdal Alkan More articles by this author , RüstemAnýl UrganRüstemAnýl Urgan More articles by this author , Mehmet Murad BasarMehmet Murad Basar More articles by this author , Zekai HalýcýZekai Halýcý More articles by this author , Mevlana Derya BalbayMevlana Derya Balbay More articles by this author , Harun UnHarun Un More articles by this author , and Hasan Tarik AtmacaHasan Tarik Atmaca More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2793AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We aimed to look into changes in testicular tissue oxidant and anti-oxidant levels in rats with experimentally induced diabetes mellitus, and also the effects of Bosentan an ET-1 receptor antagonist on the testicular tissue oxidative stress reactions. METHODS A total of 24 Albino-Wistar rats were equally allocated in 4 groups. Group 1 consisted of healthy animals as a control group, Group 2 consisted of animals with DM induced with injection of 60 mg/kg of streptozosin intraperiotenally, Groups 3 and 4 included animals with DM treated with oral Bosentan 50 mg/kg, and 100 mg/kg, respectively. At the end of 60 days of the treatment, orhiectomies were done and tissue levels of superoxyde dysmutase (SOD), glutathione synthetase (GSH) and malondialdehyde (MDA) were studied. Seminal parameters measured and testicular histopathological evaluation were also done. RESULTS Tissue levels of oxidant molecules SOD and GS were decreasesd, whereas MDA level was increased. Increase in antioxidant enzyme levels and decrease in MDA levels were most prominent and statistically significant with oral Bosertan 100 mg/kg (p<0.05). Histologically, the length of seminiferous tubules were shortened, the thickness of the basal membrane was increased, degeneration in the interstitial tissue and decrease in the number of Leydig cells were noted as well as decrease in sperm counts and motility in Group 2. Histological changes were found to be alleviated and achieved a much normal looking histology and sperm counts and motility were also found to be increased in Groups 3 and 4 in a dose dependant manner (Figure-1 and Figure-2). CONCLUSIONS Testicular effects of DM a systematic disease is profound, having a negative impact on male infertility. Bosentan prevents detoriating effects of DM on testicular tissue and improves seminal parameters in a dose dependent manner. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e986-e987 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Erdal Alkan More articles by this author RüstemAnýl Urgan More articles by this author Mehmet Murad Basar More articles by this author Zekai Halýcý More articles by this author Mevlana Derya Balbay More articles by this author Harun Un More articles by this author Hasan Tarik Atmaca More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...