MP74-06 FABRICATION OF BIOMIMETIC VASCULAR SCAFFOLDS USING VASCULAR CORROSION CASTS FOR RECONSTRUCTION OF KIDNEY TISSUES

Abstract

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation & Vascular Surgery III1 Apr 2017MP74-06 FABRICATION OF BIOMIMETIC VASCULAR SCAFFOLDS USING VASCULAR CORROSION CASTS FOR RECONSTRUCTION OF KIDNEY TISSUES Jennifer Huling, In Kap Ko, John Jackson, James Yoo, and Anthony Atala Jennifer HulingJennifer Huling More articles by this author , In Kap KoIn Kap Ko More articles by this author , John JacksonJohn Jackson More articles by this author , James YooJames Yoo More articles by this author , and Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.2174AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Vascularization is among the most pressing technical challenges facing tissue engineering of 3D organs. While small engineered tissue constructs can rely solely on vascular infiltration and diffusion from host tissues following implantation, larger avascular constructs do not survive long enough for vessel ingrowth to occur. To address this challenge, strategies for pre-vascularization of engineered constructs have been developed. To this end, we developed a simple and novel fabrication method to create biomimetic microvascular scaffolds using vascular corrosion casting as a template for pre-vascularization of engineered kidney tissue constructs. METHODS Vascular corrosion casts were made of the left kidney of adult rats. To create polycaprolactone (PCL) casts, the kidney was perfused with with 10% w/v PCL dissolved in acetone. To fabricate collagen-based vascular scaffolds from the PCL cast, the cast were dip-coated with Type 1 rat tail collagen and cross-linked. Warm acetone was used to remove the PCL casts from inside the collagen, leaving a hollow collagen vascular scaffold. To test endothelialization of the vascular scaffolds, endothelial cells labeled with green fluorescent protein (GFP) were seeded. Morphological and structural analyses were performed. RESULTS Gross and electron microscopic analysis demonstrated that polycaprolactone (PCL)-derived kidney vascular corrosion casts are able to capture the architecture of normal renal tissue and can serve as a sacrificial template for the creation of a collagen-based vascular scaffold. Histological analysis demonstrates that the collagen vascular scaffolds are biomimetic in structure and can be perfused, endothelialized, and embedded in hydrogel tissue constructs. CONCLUSIONS Our scaffold creation method is simple, cost effective, and provides a biomimetic, tissue-specific option for pre- vascularization that may be used for reconstruction of kidney tissues. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e997 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Jennifer Huling More articles by this author In Kap Ko More articles by this author John Jackson More articles by this author James Yoo More articles by this author Anthony Atala More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...