MP72-01 URINE LEVELS OF MACROPHAGE MIGRATION INHIBITORY FACTOR ARE INCREASED IN PATIENTS WITH BLADDER INFLAMMATION

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Interstitial Cystitis1 Apr 2016MP72-01 URINE LEVELS OF MACROPHAGE MIGRATION INHIBITORY FACTOR ARE INCREASED IN PATIENTS WITH BLADDER INFLAMMATION Fei Ma, Dimotrios E. Kouzoukas, Magnus M. Fall, Robert Moldwin, David M. Preston, Deborah R. Erickson, Barbara Kahn, Katherine Meyer-Siegler, and Pedro L. Vera Fei MaFei Ma More articles by this author , Dimotrios E. KouzoukasDimotrios E. Kouzoukas More articles by this author , Magnus M. FallMagnus M. Fall More articles by this author , Robert MoldwinRobert Moldwin More articles by this author , David M. PrestonDavid M. Preston More articles by this author , Deborah R. EricksonDeborah R. Erickson More articles by this author , Barbara KahnBarbara Kahn More articles by this author , Katherine Meyer-SieglerKatherine Meyer-Siegler More articles by this author , and Pedro L. VeraPedro L. Vera More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1614AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine, which appears to mediate bladder inflammation and pain in rodents (Vera et al, 2010; Kouzoukas et al, 2014). We hypothesized that urinary MIF levels would increase in patients with inflammatory diseases of the bladder, and therefore examined urine from patients with conditions including: interstitial cystitis (IC) with and without Hunner lesions, urinary tract infection (UTI), and radiation cystitis. We compared these samples to those of a control population. METHODS Urine samples were collected and studied in 3 male and 3 female groups: Specimens from males included those from patients with culture confirmed UTI, those with radiation cystitis (after undergoing radiation therapy) and a control population. Specimens from females include those from IC patients with and without Hunner lesions, and those from control subjects. Urine samples from patients with Interstitial Cystitis met NIDDK diagnostic criteria. All IC patients were symptomatic at the time of urine collection. Urinary MIF and Creatinine levels were assayed using commercially available kits (R&D; Enzo). RESULTS MIF levels were significantly increased in the urine of UTI patients (>4-fold; N=51) or radiation cystitis patients (10-fold; N=18) when compared to controls (N=117), either as total concentration or normalized to creatinine levels (Table 1). Similarly, MIF levels were significantly increased (5-fold; Table 2) in the urine of IC patients with Hunner lesions (N=47) when compared to female controls (N=102). Urine MIF levels in IC patients without Hunner lesions (N=63) were not different from controls. CONCLUSIONS Urinary MIF is increased in a variety of bladder inflammatory conditions including interstitial cystitis with Hunner lesions, UTI and radiation cystitis. Conversely, urine MIF levels from IC patients without Hunner lesions are similar to controls. These findings may represent differences in pathogenesis between these conditions and may serve as a marker to distinguish them from each other. MIF may be useful for diagnosis or prognosis of IC patients with Hunner lesions and may represent a novel therapeutic target for reducing bladder pain in IC and other bladder inflammatory disorders. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e952 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Fei Ma More articles by this author Dimotrios E. Kouzoukas More articles by this author Magnus M. Fall More articles by this author Robert Moldwin More articles by this author David M. Preston More articles by this author Deborah R. Erickson More articles by this author Barbara Kahn More articles by this author Katherine Meyer-Siegler More articles by this author Pedro L. Vera More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...