MP71-07 SERUM CORE FUCOSYLATED N-GLYCAN CARRYING IMMUNOGLOBRIN IS A DIAGNOSTIC AND PRE-OPERATIVE PROGNOSTIC MARKER OF UPPER TRACT UROTHELIAL CARCINOMA

Abstract

You have accessJournal of UrologyBladder Cancer: Upper Tract Transitional Cell Carcinoma I1 Apr 2017MP71-07 SERUM CORE FUCOSYLATED N-GLYCAN CARRYING IMMUNOGLOBRIN IS A DIAGNOSTIC AND PRE-OPERATIVE PROGNOSTIC MARKER OF UPPER TRACT UROTHELIAL CARCINOMA Toshikazu Tanaka, Yuki Tobisawa, Shingo Hatakeyama, Kazuyuki Mori, Yasuhiro Hashimoto, Takuya Koie, Chikara Ohyama, and Tohru Yoneyama Toshikazu TanakaToshikazu Tanaka More articles by this author , Yuki TobisawaYuki Tobisawa More articles by this author , Shingo HatakeyamaShingo Hatakeyama More articles by this author , Kazuyuki MoriKazuyuki Mori More articles by this author , Yasuhiro HashimotoYasuhiro Hashimoto More articles by this author , Takuya KoieTakuya Koie More articles by this author , Chikara OhyamaChikara Ohyama More articles by this author , and Tohru YoneyamaTohru Yoneyama More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.2263AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Although hematuria and urine cytology is a powerful indicator for diagnosis of upper tract urothelial carcinoma (UTUC), there is a need to identify more powerful predictive biomarkers of UTUC to improve diagnostic accuracy. Prognostic biomarkers were also required to decide on therapeutic strategy of UTUC. The use of N-glycans of immunoglobrins (Igs) as a biomarkers of UTUC has not yet been tested. In the present study, we performed serum N-glycomics between healthy volunteer (HLT) and UTUC patients and evaluated its potential as a diagnostic and prognostic serum-based biomarkers of UTUC. METHODS N-glycomics in Igs fraction were performed in randomly selected 78 HLT and 73 UTUC (preoperative serum, n=52 and post-operative serum, n=21, respectively) patients. The putative structure of N-glycans was analyzed by MALDI-TOF-MS analysis. To confirm the result of N-glycomics of Igs, We performed lectin-array analysis utilizing recombinant lectin-array chip and evanescent fluorescence scanner. RESULTS N-glycomics of Igs reveled that preoperative serum core fucosylated biantennary (m/z 1590, 2073, and 2524) and core fucosylated bisecting (m/z 1647, 1793, 2955 and 2727) N-glycans of Igs levels in UTUC patients was significantly higher in HLT group. Receiver-operating characteristic area under the curve (AUC) for prediction of UTUC in significant core fucoslyalted N-glycans was 0.7290-0.8728 in contrast to hematuria positive status (AUC 0.8627) and urine cytology class =IV (AUC 0.7745), respectively. In post-operative serum analysis, we found that the level of serum core fucosylated N-glycans in cancer death group was tend to higher than that of surviving group. Furthermore, lectin-array analysis revealed that fucoslyted IgG (Fuc-IgG) level of UTUC was tend to higher than HLT. In post-operative serum analysis, we also found serum fuc-IgG levels in cancer death group was significantly higher than that of surviving group. CONCLUSIONS The serum core fucosylated N-glycans levels and Fuc-IgG levels may be a promising diagnostic and post-operative prognostic markers of UTUC. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e947-e948 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Toshikazu Tanaka More articles by this author Yuki Tobisawa More articles by this author Shingo Hatakeyama More articles by this author Kazuyuki Mori More articles by this author Yasuhiro Hashimoto More articles by this author Takuya Koie More articles by this author Chikara Ohyama More articles by this author Tohru Yoneyama More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...