MP69-20 HIGH PREDICTION RATE OF PROSTATE CANCER WITH MINIMALLY INVASIVE OLIGOFISH TEST

Abstract

You have accessJournal of UrologyProstate Cancer: Detection & Screening V1 Apr 2014MP69-20 HIGH PREDICTION RATE OF PROSTATE CANCER WITH MINIMALLY INVASIVE OLIGOFISH TEST Jason Hafron, Mike Gildea, Josh Kahl, Rima Aljundi, Jackie Day, Paola Yumpo, Peggy Morgan, Michael Lutz, J. Rene Frontera, Carrie Ng, Jacqueline Dion, Chris Young, Shannon Knuth, Robert Di Loreto, Kenneth Kernen, and Joan Aurich-Costa Jason HafronJason Hafron More articles by this author , Mike GildeaMike Gildea More articles by this author , Josh KahlJosh Kahl More articles by this author , Rima AljundiRima Aljundi More articles by this author , Jackie DayJackie Day More articles by this author , Paola YumpoPaola Yumpo More articles by this author , Peggy MorganPeggy Morgan More articles by this author , Michael LutzMichael Lutz More articles by this author , J. Rene FronteraJ. Rene Frontera More articles by this author , Carrie NgCarrie Ng More articles by this author , Jacqueline DionJacqueline Dion More articles by this author , Chris YoungChris Young More articles by this author , Shannon KnuthShannon Knuth More articles by this author , Robert Di LoretoRobert Di Loreto More articles by this author , Kenneth KernenKenneth Kernen More articles by this author , and Joan Aurich-CostaJoan Aurich-Costa More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.2239AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Detection of prostate cancer is currently performed by transrectal biopsies, sampling only a small part of the gland and resulting in a high rate of false negatives. We used OligoFISH probes for aneuploidy detection in voided urine from 50 patients obtained after digital rectal exam (DRE) with pressure. Our goal was to find a prostate cancer screening method better than PSA and prostate biopsy. Aneuploidy, an abnormal number of chromosomes, is a common hallmark of cancer, including prostate cancer. We analyzed the karyotype of 100 cases of prostate carcinoma from Mitelman’s database and other bibliography and identified chromosomes Y, 7, 10, 20, 6, 8, 13, 16 and 18 to be polysomic in most prostate carcinoma cases. METHODS Fifty patients scheduled to undergo prostate biopsy were recruited. Subjects signed informed consent and received a DRE with pressure by the urologist, the first urine was collected and slides were prepared. We have shown previously that this collection method yields a sample containing 65% prostate cells. Slides were fixed in Carnoy and aged on a hot plate before undergoing our proprietary room temperature pretreatments and 10 minute hybridization with two 4-color OligoFISH probe panels covering 8 chromosomes: Y, 6, 7, 8, 10, 16, 18 and 20. After a room temperature wash, 500 cells were scored for each set of probes. Results were compared to biopsy and follow-up results. RESULTS Both the A (6, Y, 10, 8) and B (7, 16, 18, 20) probe panels were excellent in differentiating benign vs. cancer. The area under the ROC curve (AUC) was 0.88 and 0.89, respectively. Both panels had exceptional clinical sensitivity of 96% and specificity of 75 and 88%, respectively. In differentiating low grade (Gleason Score ≤ 6) from high grade (Gleason score > 6) lesions, the AUC was 0.61 and 0.59, respectively. Both panels had specificity of 69 and 71% and sensitivity of 60 and 71% between low grade and high grade lesions. As seen in the literature, chromosome Y gains were the most informative in identifying high grade lesions. CONCLUSIONS Although this study includes a small sample (50 individuals), the results are very encouraging with regard to pursuing a full clinical trial. Use of this minimally invasive test could dramatically decrease the number of transrectal biopsies performed on men with a benign condition of the prostate. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e822 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Jason Hafron More articles by this author Mike Gildea More articles by this author Josh Kahl More articles by this author Rima Aljundi More articles by this author Jackie Day More articles by this author Paola Yumpo More articles by this author Peggy Morgan More articles by this author Michael Lutz More articles by this author J. Rene Frontera More articles by this author Carrie Ng More articles by this author Jacqueline Dion More articles by this author Chris Young More articles by this author Shannon Knuth More articles by this author Robert Di Loreto More articles by this author Kenneth Kernen More articles by this author Joan Aurich-Costa More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...