MP68-09 EULERIAN VIDEO MAGNIFICATION: A NOVEL TECHNIQUE FOR IMPROVED SPERM SELECTION IN MEN WITH SEVERE OLIGOASTHENOSPERMIA

Abstract

You have accessJournal of UrologyInfertility: Evaluation1 Apr 2014MP68-09 EULERIAN VIDEO MAGNIFICATION: A NOVEL TECHNIQUE FOR IMPROVED SPERM SELECTION IN MEN WITH SEVERE OLIGOASTHENOSPERMIA Gavin Langille, James Dupree, Jason Kovac, Ranjith Ramasamy, Dolores Lamb, and Larry Lipshultz Gavin LangilleGavin Langille More articles by this author , James DupreeJames Dupree More articles by this author , Jason KovacJason Kovac More articles by this author , Ranjith RamasamyRanjith Ramasamy More articles by this author , Dolores LambDolores Lamb More articles by this author , and Larry LipshultzLarry Lipshultz More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.2188AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The absence of sufficient motile sperm in samples used for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) can present a serious clinical problem for infertility specialists wishing to select viable sperm. Identification of optimal quality sperm can be especially challenging in men with oligoasthenospermia. Eulerian Video Magnification (EVM) is a technique that provides an opportunity to detect subtle movements in video feeds not appreciable to the unaided human eye. EVM is a computational algorithm that uses spatial and temporal variations present in video sources to enhance, in a spatially-multiscale manner, the variation of individual pixel values over time. In patients whose semen samples demonstrate significantly impaired motility by traditional light microscopy, EVM could be used to select viable motile sperm that would have been otherwise identified as non-motile using traditional light microscopic visualization. METHODS Using traditional light microscopy with 400x magnification, semen analysis (SA) samples from men demonstrating severe oligoasthenospermia (density < 1 million/ml and motility < 20%) were investigated. A high definition (HD) video camera was used to record video footage from three high-powered fields (HPF) per SA. Each video recording was scored twice for motility, first using the raw HD footage, and second after the video was processed using EVM previously developed at the Massachusetts Institute of Technology (http://people.csail.mit.edu/mrub/vidmag). Quantitation of motile sperm in each video recording was compared between the raw and EVM-enhanced images. RESULTS Video from eleven SA samples with three recordings each were analyzed. A total of 442 sperm were recorded across all samples. After processing with EVM, the number of additionally recognized motile/viable sperm increased by 14% (p<0.05). CONCLUSIONS EVM is a novel technique to identify viable sperm that would not have been recognized using traditional light microscopy. Using a micro-locator and micro-pipette, these sperm can then be retrieved for IVF-ICSI. Our data suggests that an additional 14% of retrieved ova can then be inseminated. Further studies are underway to refine this technique and to test the efficacy of the additional EVM-identified sperm in improving IVF-ICSI outcomes for the oligoasthenospermic patient. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e801 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Gavin Langille More articles by this author James Dupree More articles by this author Jason Kovac More articles by this author Ranjith Ramasamy More articles by this author Dolores Lamb More articles by this author Larry Lipshultz More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...