MP68-05 COMPARISON OF THE EFFECTS OF BRAIN-DERIVED NEUROTROPHIC FACTOR (BDNF) AND NERVE GROWTH FACTOR (NGF) INHIBITION ON BLADDER FUNCTION OF MICE WITH SPINAL CORD INJURY (SCI)

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology II1 Apr 2016MP68-05 COMPARISON OF THE EFFECTS OF BRAIN-DERIVED NEUROTROPHIC FACTOR (BDNF) AND NERVE GROWTH FACTOR (NGF) INHIBITION ON BLADDER FUNCTION OF MICE WITH SPINAL CORD INJURY (SCI) Naoki Wada, Takahiro Shimizu, Shun Takai, Nobutaka Shimizu, Pradeep Tyagi, William de Groat, Anthony Kanai, Hidehiro Kakizaki, and Naoki Yoshimura Naoki WadaNaoki Wada More articles by this author , Takahiro ShimizuTakahiro Shimizu More articles by this author , Shun TakaiShun Takai More articles by this author , Nobutaka ShimizuNobutaka Shimizu More articles by this author , Pradeep TyagiPradeep Tyagi More articles by this author , William de GroatWilliam de Groat More articles by this author , Anthony KanaiAnthony Kanai More articles by this author , Hidehiro KakizakiHidehiro Kakizaki More articles by this author , and Naoki YoshimuraNaoki Yoshimura More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1342AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES BDNF and NGF are reportedly involved in changes in neural pathways to induce lower urinary tract (LUT) dysfunction such as detrusor overactivity (DO) and inefficient voiding following SCI. However, it has not been well clarified how these two growth factors differentially affect storage and voiding functions after SCI. Therefore, we investigated the effects of anti-NGF or anti-BDNF antibody treatment on cystometric parameters in SCI mice. METHODS SCI was produced by complete transection of the Th8/9 spinal cord in female C57BL/6N mice. Three weeks later, an osmotic pump was placed subcutaneously to administer vehicle (group A), 10µg/kg/hr of anti-BDNF antibody (group B) or 10µg/kg/hr of anti-NGF antibody (group C) for 1 week. Four weeks after transection, SCI mice were evaluated using continuous or single-filling cystometry under an awake condition. RESULTS In continuous cystometry, voiding efficiency was significantly increased in the groups B (21%) and C (26%) compared to the group A (14%), and postvoid residual volume (PVR) was lower in the group C (0.14ml) vs. the group A (0.25ml). There was also a tendency of the reduction in non-voiding contractions (NVC) in the group C without statistical significance. Micturition pressure (MP) or intercontraction intervals were not different among the groups. In single cystometry, voided volume (0.085 vs. 0.038 ml) and voiding efficiency (42% vs. 18%) in the group B were significantly increased compared to the group A, and PVR of the group C was lower than that of the group A (0.11 vs. 0.15 ml). MP, maximum cystometric capacity or NVC was not different among groups. CONCLUSIONS Both anti-BDNF and anti-NGF antibody treatments improved voiding dysfunction as shown by increased voiding efficiency due to increased voided volume or decreased PVR in SCI mice. In addition, the anti-NGF, but not anti-BDNF, treatment marginally improved NVCs. These results suggest that BDNF and NGF are involved in SCI-induced voiding dysfunction resulting in inefficient voiding, but have minor roles in storage dysfunction to induce DO in mice, in contrast to the previous findings in SCI rats showing the greater contribution of bladder NGF or BDNF overexpression to DO. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e890-e891 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Naoki Wada More articles by this author Takahiro Shimizu More articles by this author Shun Takai More articles by this author Nobutaka Shimizu More articles by this author Pradeep Tyagi More articles by this author William de Groat More articles by this author Anthony Kanai More articles by this author Hidehiro Kakizaki More articles by this author Naoki Yoshimura More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...