MP68-19 MESENCHYMAL STEM CELLS ATTENUATES KETAMINE-INDUCED INTERSTITIAL CYSTITIS

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology II1 Apr 2016MP68-19 MESENCHYMAL STEM CELLS ATTENUATES KETAMINE-INDUCED INTERSTITIAL CYSTITIS Aram Kim, Hwan Yeul Yu, Jung-Hyun Shin, Dong-Myung Shin, and Myung-Soo Choo Aram KimAram Kim More articles by this author , Hwan Yeul YuHwan Yeul Yu More articles by this author , Jung-Hyun ShinJung-Hyun Shin More articles by this author , Dong-Myung ShinDong-Myung Shin More articles by this author , and Myung-Soo ChooMyung-Soo Choo More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1356AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Ketamine has been abused as a hallucinogenic drug and is increasingly being used by young people. Ketamine abuse promotes the development of lower urinary tract symptoms that resemble interstitial cystitis (IC). The present study evaluated the therapeutic effect of human umbilical cord blood-derived mesenchymal stem cells (MSCs) in a ketamine-induced cystitis (KC) rat model. METHODS Thirty female 10-week-old Sprague-Dawley rats were intravenously injected with ketamine (25 mg/kg) or phosphate-buffered saline (PBS, sham) five times per week for 2 weeks. One week after final administration of ketamine, one million of UCB-MSCs (n=10, KC+MSC) or PBS (n=10, KC) were injected into the submucosal layer of the anterior wall and dome of the bladder. The therapeutic effect of UCB-MSCs was examined by cystometry, histological and gene expression analysis. RESULTS Rats in the KC group exhibited irregular voiding frequency, increased maximum contraction pressure and decreased inter-contraction intervals, compared with sham group (118.8 ± 34.3 vs. 306.5 ± 138.5 seconds, respectively; p<0.05). A single injection of MSCs significantly improved most of voiding parameters by increased the inter-contraction interval and decreased the maximum contraction pressure and the proportion of rats with detrusor overactivity. The bladder of KC group rats were characterized with severe mast-cell infiltration, fibrosis, and apoptosis, however little damage in urothelium. Gene expression analysis indicated that tumor necrosis factor-alpha (TNF?) and C-X-C motif chemokine 10 (CXCL10) were up-regulated in KC bladder. MSC treatment significantly reversed the histological and gene expression alternations characteristic for KC bladder. Particularly, the treatment of N-acetylcysteine (NAC), an anti-fibrosis compound alleviated the KC bladder, similarly to MSC therapy. CONCLUSIONS This study for the first time demonstrate that MSC therapy is beneficial to cure KC using an animal model. Our findings not only provide the basis for clinical trials of MSC therapy to painful bladder conditions such as KC and IC. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e896-e897 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Aram Kim More articles by this author Hwan Yeul Yu More articles by this author Jung-Hyun Shin More articles by this author Dong-Myung Shin More articles by this author Myung-Soo Choo More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...