MP67-10 IDENTIFICATION OF URINARY MACROPHAGE-RELATED FACTORS SPECIFIC TO PATIENTS WITH CALCIUM OXALATE STONES USING MULTIPLEX ANALYSIS

Abstract

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II1 Apr 2016MP67-10 IDENTIFICATION OF URINARY MACROPHAGE-RELATED FACTORS SPECIFIC TO PATIENTS WITH CALCIUM OXALATE STONES USING MULTIPLEX ANALYSIS Atsushi Okada, Kazumi Taguchi, Yasuhiro Fujii, Shuzo Hamamoto, Masahito Hirose, Yasunori Itoh, Keiichi Tozawa, Shoichi Sasaki, Yutaro Hayashi, Kenjiro Kohri, and Takahiro Yasui Atsushi OkadaAtsushi Okada More articles by this author , Kazumi TaguchiKazumi Taguchi More articles by this author , Yasuhiro FujiiYasuhiro Fujii More articles by this author , Shuzo HamamotoShuzo Hamamoto More articles by this author , Masahito HiroseMasahito Hirose More articles by this author , Yasunori ItohYasunori Itoh More articles by this author , Keiichi TozawaKeiichi Tozawa More articles by this author , Shoichi SasakiShoichi Sasaki More articles by this author , Yutaro HayashiYutaro Hayashi More articles by this author , Kenjiro KohriKenjiro Kohri More articles by this author , and Takahiro YasuiTakahiro Yasui More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1326AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We previously demonstrated the involvement of inflammatory macrophages (M1-Mϕ) and anti-inflammatory macrophages (M2-Mϕ) in the calcium oxalate (CaOx) kidney stone formation process by basic studies using an animal model. However, in clinical medicine, the correlations between stone formation risk and Mϕ activity have not been examined. The aim of the present study was to analyze the characteristics of CaOx stone formation factors and detect the urinary Mϕ-related factors specific to stone formation. METHODS Subjects were 249 men aged 20?79 years old who visited Nagoya City University Hospital. Of them, 111 had no history of stone formation (non-stone formers [NSF]), while 130 had a history of CaOx stone formation (stone formers [SF]): 41 had experienced one episode of stone formation and 89 had a history of recurrent stone formation. All patients had normal urinalysis results and denied a history of malignancy, collagen vascular disease, or the use of immunosuppressive or steroid medications. Using 25 µL of spot urine, we determined the concentration of 18 urinary candidate proteins using multiplex (MP) analysis on a MagPix® system. For the factors that demonstrated significant differences between NSF and SF, we performed multi- and univariate analyses for patient age, body mass index, blood and urine biochemistry data, and MP analysis data and calculated the odds ratio and 95% confidence interval of the CaOx SF using logistic regression analysis. For each parameter, the median was used as the cut-off value. RESULTS We found no significant differences in the background data or urine biochemistry between NSF and SF. The two-stage MP analyses demonstrated eight factors with significant differences between the two groups. Logistic regression analyses revealed that lower values of interleukin (IL)-4 (≤4.4 pg/mL), IL-1a (≤7.9 pg/mL), and granulocyte colony-stimulating factor (≤14.5 pg/mL) were statistically significant independent factors predictive of stone formation. Furthermore, on the validation analyses, we found significantly lower values than NSF group regardless of first time or recurrence. CONCLUSIONS CaOx SF show a significantly lower M2-Mϕ activity-related urinary IL-4 level than NSF. In CaOx SF, decreased function of M2-Mϕ, including phagocytosis and processing of CaOx crystals, may play a more important role in the stone formation process than urinary biochemical abnormalities. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e884 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Atsushi Okada More articles by this author Kazumi Taguchi More articles by this author Yasuhiro Fujii More articles by this author Shuzo Hamamoto More articles by this author Masahito Hirose More articles by this author Yasunori Itoh More articles by this author Keiichi Tozawa More articles by this author Shoichi Sasaki More articles by this author Yutaro Hayashi More articles by this author Kenjiro Kohri More articles by this author Takahiro Yasui More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...