MP66-19 REDUCED AUTOPHAGY LEVELS ARE ASSOCIATED WITH A HIGHER GLEASON SCORE, TUMOR STAGE AND AN INCREASED RATE OF PROSTATE CANCER SPECIFIC DEATH

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research V1 Apr 2015MP66-19 REDUCED AUTOPHAGY LEVELS ARE ASSOCIATED WITH A HIGHER GLEASON SCORE, TUMOR STAGE AND AN INCREASED RATE OF PROSTATE CANCER SPECIFIC DEATH Ashkan Mortezavi, Souzan Salemi, Niels Rupp, Thomas Hermanns, Tullio Sulser, Peter J. Wild, and Daniel Eberli Ashkan MortezaviAshkan Mortezavi More articles by this author , Souzan SalemiSouzan Salemi More articles by this author , Niels RuppNiels Rupp More articles by this author , Thomas HermannsThomas Hermanns More articles by this author , Tullio SulserTullio Sulser More articles by this author , Peter J. WildPeter J. Wild More articles by this author , and Daniel EberliDaniel Eberli More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2372AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Autophagy is a catabolic cellular process used for degradation of cytoplasmic organelles and preservation of cell viability. Data regarding the role of autophagy in treatment naive prostate cancer (PCa) is sparse. Therefore we aimed to analyze the level of autophagy in benign and malignant prostate tissue and to evaluate the prognostic properties for patients with PCa. METHODS A Tissue microarray containing 479 formalin-fixed, paraffin-embedded prostate tissue cores was stained immunohistochemically for major autophagy proteins ATG5, Beclin1 and LC3b: 41 normal prostate tissues, 382 primary adenocarcinomas (ADC) and 56 prostate cancer metastases or castration resistant prostate cancers. Immunoreactivity was semiquantitatively scored and correlated with pathologic and clinical parameters including survival. RESULTS LC3b expression was significantly upregulated in PCa, especially in metastatic and castration-resistant prostate cancer samples compared to normal prostate tissue (p=0.002). Evaluation of expression in ADCs revealed an inverse association with preoperative serum PSA level (p=0.047), pT-stage (0.033) and Gleason Score (p=0.026). Negative LC3b expression was identified as a novel predictor of prostate cancer specific death after radical prostatectomy (p=0.02). A significant association of ATG-5 and Beclin1 with LC3b expression could be noticed, but no link with clincopathologic parameters. CONCLUSIONS A lower LC3b expression in prostate cancer tissue is significantly associated with a higher rate of prostate cancer specific death. Furthermore, there is a significant association of a lower expression in tumors with extraprostatic extension and higher Gleason Scores. This is the first report evaluating autophagy expression levels and their association with the clinical course in prostate cancer patients. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e823 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ashkan Mortezavi More articles by this author Souzan Salemi More articles by this author Niels Rupp More articles by this author Thomas Hermanns More articles by this author Tullio Sulser More articles by this author Peter J. Wild More articles by this author Daniel Eberli More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...