MP66-13 14-3-3 EPSILON MAY PLAY AN IMPORTANT ROLE IN TESTICULAR GERM CELL APOPTOSIS: A FUNCTIONAL PROTEOMIC STUDIES OF EXPERIMENTAL VARICOCELE

Abstract

You have accessJournal of UrologyInfertility: Basic Research, Physiology & Pathophysiology1 Apr 2014MP66-13 14-3-3 EPSILON MAY PLAY AN IMPORTANT ROLE IN TESTICULAR GERM CELL APOPTOSIS: A FUNCTIONAL PROTEOMIC STUDIES OF EXPERIMENTAL VARICOCELE Yan Zhang, Zhongmin Yuan, Qingqing He, Hao Zhang, Xiaopeng Liu, Jinming Di, Liaoyuan Li, Xiaojian Yang, and Xin Gao Yan ZhangYan Zhang More articles by this author , Zhongmin YuanZhongmin Yuan More articles by this author , Qingqing HeQingqing He More articles by this author , Hao ZhangHao Zhang More articles by this author , Xiaopeng LiuXiaopeng Liu More articles by this author , Jinming DiJinming Di More articles by this author , Liaoyuan LiLiaoyuan Li More articles by this author , Xiaojian YangXiaojian Yang More articles by this author , and Xin GaoXin Gao More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.2060AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The objective of this study was toidentify the differential proteins expressing in experimental varicoceleby proteomics technique, and investigate the role of 14-3-3∈ in testicular germ cell apoptosis. METHODS Sixteen male Wistar rats were randomly assigned into sham operation and varicocele groups. Left testes were harvested 30 days after the induction of varicocele. Proteomics assaywas performed to determine the expression level of protein of interest. Among the 21 proteins identified expressing differently between sham an varicocele, the levels of 14-3-3∈, Bax, twokey regulators for survival and apoptosis, were detected in germ cellsby western blotting and their location was determined by Immunohistochemistry (IHC). TUNEL method was used to analyze the apoptotic rate of germ cells. In vitro study was also applied to confirm whether silencing 14-3-3∈ can induce apoptosis in GC-1 spg cells. RESULTS Compared with control group, the 14-3-3∈ protein expression was significantly declined while Baxwasincreased in experimental group. The apoptosis index (AI) in experimental groupwas obviously higher than that in sham group. Knockdowning 14-3-3∈ by siRNA greatly induced apoptosis of GC-1 spg cell, with the up-regulation of cleaved caspase-3. CONCLUSIONS Our in vivo study demonstratedthat varicocele caused a significant down-regulation of 14-3-3∈ butup-regulation of Bax, which locally occurred in theapoptoticspermatogenic cell. In vitro study supported that downregulation of 14-3-3∈ contributed to germ cell apoptosis. Collectively, the down-regulation of 14-3-3∈ or up-regulation of Baxwas tightly correlated with germ cell apoptosis induced by varicocele. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e745 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yan Zhang More articles by this author Zhongmin Yuan More articles by this author Qingqing He More articles by this author Hao Zhang More articles by this author Xiaopeng Liu More articles by this author Jinming Di More articles by this author Liaoyuan Li More articles by this author Xiaojian Yang More articles by this author Xin Gao More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...