MP65-12 ORGANOID 3D CULTURE SYSTEM FOR BLADDER CANCER MODELING

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology III1 Apr 2018MP65-12 ORGANOID 3D CULTURE SYSTEM FOR BLADDER CANCER MODELING Victor Romanov, William Berg, Terry Whyard, Wei Lee, Olga Povcher, Daniel Rittenberg, and Wayne Waltzer Victor RomanovVictor Romanov More articles by this author , William BergWilliam Berg More articles by this author , Terry WhyardTerry Whyard More articles by this author , Wei LeeWei Lee More articles by this author , Olga PovcherOlga Povcher More articles by this author , Daniel RittenbergDaniel Rittenberg More articles by this author , and Wayne WaltzerWayne Waltzer More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2078AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The absence of in vitro bladder cancer models recapitulating the diversity of urothelial tumors hinders our understanding of the disease pathogenesis and postpones development of the effective therapeutic modalities. Recent achievement in the development of 3D organoid systems enable stem or progenitor cells to form diverse, multicellular structures, that can self-renew, self-organize, and differentiate. Development of 3D organoid culture from bladder urothelium provides an unique model for studying cancer. These structures can be genetically modified to mimic individual steps of bladder cancer tumorigenesis. 3D spheroids derived from bladder tumors can be directly used in preclinical applications METHODS We developed 3D organoid cultures of urothelial cells that derived from experimental animals (mice) and human (ureter and bladder). Optimal conditions for preferential organoid development were determined. In addition 3D spheroids were established from surgical samples of papillary tumors. These structures were analyzed morphologically and biochemically. Tumor derived spheroids were also used for anti-cancer drug sensitivity testing. RESULTS We established procedure for generation organoids derived from single cell from bladder or ureter. These structures were characterized immunocytochemically and was shown that different types of urothelial cells were present. For bladder cancer derived organoid we used different preparative procedure based on the partial digestion of the tissue. In this case spheroids were formed from cellular conglomerates. Using tumor derived spheroids in drug sensitivity assay we have showed that potential drug-Clostridium perfringens toxin is superior as compared to currently exploited chemotherapeutical modalities for intravesical treatment. CONCLUSIONS Organoid 3D urothelial culture can be used for studying tumorigenesis in bladder cancer and for preclinical testing of potential chemotherapeutic agents. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e863 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Victor Romanov More articles by this author William Berg More articles by this author Terry Whyard More articles by this author Wei Lee More articles by this author Olga Povcher More articles by this author Daniel Rittenberg More articles by this author Wayne Waltzer More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...