MP62-13 NOVEL IN VIVO MODEL FOR COMBINATORIAL FLUORESCENCE LABELING IN MOUSE PROSTATE

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research & Pathophysiology I1 Apr 2016MP62-13 NOVEL IN VIVO MODEL FOR COMBINATORIAL FLUORESCENCE LABELING IN MOUSE PROSTATE Xiaolan Fang, Michael B. Rothberg, Kenneth Gyabaah, Bita Nick Kholgh, J. Marc Cline, and K.C. Balaji Xiaolan FangXiaolan Fang More articles by this author , Michael B. RothbergMichael B. Rothberg More articles by this author , Kenneth GyabaahKenneth Gyabaah More articles by this author , Bita Nick KholghBita Nick Kholgh More articles by this author , J. Marc ClineJ. Marc Cline More articles by this author , and K.C. BalajiK.C. Balaji More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.921AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Currently, few animal models exist to study the cellular origin of normal or neoplastic development in the prostate by tracing multiple cell lineages in vivo. We previously described a mouse model that expresses fluorescent proteins (XFPs) for normal tissue, benign hyperplasia, and primary neoplasia in the prostate. We have since developed this model to demonstrate more advanced forms of prostate cancer to facilitate the treatment of heterogeneous prostate diseases by targeting individual cell lineages. METHODS Using an in vivo stochastic fluorescent protein combinatorial strategy, XFP signals were expressed specifically in the prostate of: (1) Protein Kinase D1 (PKD1) knock-out, K-Ras G12D knock-in, (2) Phosphatase and tensin homolog (PTEN) knock-out, K-Ras G12D knock-in, and (3) K-Ras G12D knock-in, PTEN PKD1 knock-out triple mutant mice under the control of the prostate-specific Probasin (PB) promoter. RESULTS In vivo XFP signals were observed in the prostate of (1) PKD1 knock-out, K-Ras G12D knock-in, (2) PTEN knock-out, K-Ras G12D knock-in, and (3) K-Ras G12D knock-in, PTEN PKD1 knock-out triple mutant mice, which developed prostate cancer sporadically invading the urethra and micro-metastasis to the lung, in situ carcinoma aggressively invading the urethra, and a highly aggressive prostate cancer resulting in early animal death, respectively. The unique expression pattern of XFPs in neoplastic tissue indicated the clonal origin of cancer cells in the prostate. CONCLUSIONS This transgenic mouse model demonstrates combinatorial fluorescent protein expression in locally aggressive and metastatic cancerous prostatic tissues. This novel prostate-specific fluorescent-labeled mouse model, which we named Prorainbow, could be useful in studying benign and malignant pathology of the prostate. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e817 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Xiaolan Fang More articles by this author Michael B. Rothberg More articles by this author Kenneth Gyabaah More articles by this author Bita Nick Kholgh More articles by this author J. Marc Cline More articles by this author K.C. Balaji More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...