MP61-03 A BIOFUNCTIONAL VASCULAR SCAFFOLD FOR REPLACING SMALL-DIAMETER BLOOD VESSELS

Abstract

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation & Vascular Surgery I1 Apr 2018MP61-03 A BIOFUNCTIONAL VASCULAR SCAFFOLD FOR REPLACING SMALL-DIAMETER BLOOD VESSELS Young Min Ju, Ickhee Kim, John Jackson, Anthony Atala, James Yoo, and Sang Jin Lee Young Min JuYoung Min Ju More articles by this author , Ickhee KimIckhee Kim More articles by this author , John JacksonJohn Jackson More articles by this author , Anthony AtalaAnthony Atala More articles by this author , James YooJames Yoo More articles by this author , and Sang Jin LeeSang Jin Lee More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1982AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Disease of the renal vascular system has resulted in a clear clinical need for the development of functional vascular substitutes. We have previously fabricated a vascular scaffold by electrospinning of poly(e-caprolactone) (PCL) combined with collagen. In this study, we hypothesized that multiple bioconjugation of endothelial progenitor cells (EPC)/endothelial cell (EC) specific antibodies and anti-thrombogenic agents onto the vascular scaffold could facilitate in situ endothelialization while preventing blood clotting. METHODS Vascular scaffolds were fabricated by electrospinning of poly(e-caprolactone) (PCL) combined with collagen. Bioconjugation of endothelial progenitor cells (EPC)/endothelial cell (EC) specific antibodies and anti-thrombogenic agents were performed onto the vascular scaffold. The bioconjugated scaffolds were implanted in a sheep model of vascular defect. RESULTS The biofunctionalized scaffolds conjugated with EPC/EC-specific antibodies and heparin were able to achieve effective EPC/EC capturing and anti-thrombogenesis. In vivo experiment showed that these vascular scaffolds maintained a high degree of patency and structural integrity without eliciting a histologic inflammatory response over the course of 6-month period in sheep. Moreover, the matured EC coverage on the lumen and smooth muscle layer were observed at 6 months after implantation. CONCLUSIONS We demonstrate that the generation of the biofunctional vascular scaffold, along with existing tools for selection and directed immobilization of EPC/EC-specific antibodies and anti-thrombogenic agent, could provide the necessary components for successful tissue engineering of small diameter blood vessels in the renal vascular system. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e824 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Young Min Ju More articles by this author Ickhee Kim More articles by this author John Jackson More articles by this author Anthony Atala More articles by this author James Yoo More articles by this author Sang Jin Lee More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...