MP60-14 A CELLULAR ATLAS OF THE NORMAL ADULT HUMAN FEMALE URETHRA

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP60-14 A CELLULAR ATLAS OF THE NORMAL ADULT HUMAN FEMALE URETHRA John Lafin, Alicia Malewska, Philippe Zimmern, Maude Carmel, Gary Lemack, Douglas Strand, and Ramy Goueli John LafinJohn Lafin More articles by this author , Alicia MalewskaAlicia Malewska More articles by this author , Philippe ZimmernPhilippe Zimmern More articles by this author , Maude CarmelMaude Carmel More articles by this author , Gary LemackGary Lemack More articles by this author , Douglas StrandDouglas Strand More articles by this author , and Ramy GoueliRamy Goueli More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003318.14AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Urinary voiding dysfunction is highly prevalent in aging women. A deeper understanding of the cellular composition of female urethra in the non-diseased state could provide insights into the etiology of dysfunction. Single cell RNA sequencing was performed on female urethra to generate a cellular atlas. METHODS: Urethra from female organ donors aged 18-45 (N=4) was collected fresh from surgery and enzymatically digested into a single cell suspension. Single cell RNA sequencing was performed using 10x Genomics V3 chemistry. Data were analyzed in Seurat for cell type annotation. Immunohistochemical validation of cell types was performed on FFPE sections using differentially expressed genes from clustered data. RESULTS: The female proximal urethral lumen is lined with a stratified urothelium composed of KRT5+/KRT14- basal cells with KRT13+ hillock epithelia and KRT4+ luminal epithelia. Ducts emerging from dorsal urethra (Skene's glands) contain basal and club cells similar to prostatic urethra. The urethral lumen is surrounded by two discrete layers of fibroblasts (peri-urethral and interstitial) and circumferential smooth muscle and skeletal muscle. CONCLUSIONS: The identification of 1) specialized fibroblasts surrounding the urethra, and 2) club cells in dorsal ducts could yield new insight into the regulation of infection, inflammation and obstruction, which are the putative etiologies of most urethral dysfunction. Source of Funding: DK115477, DK104310, DK129994 © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e848 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information John Lafin More articles by this author Alicia Malewska More articles by this author Philippe Zimmern More articles by this author Maude Carmel More articles by this author Gary Lemack More articles by this author Douglas Strand More articles by this author Ramy Goueli More articles by this author Expand All Advertisement PDF downloadLoading ...