MP60-08 CHRONIC PSYCHOLOGICAL STRESS INDUCES CHANGES IN BLADDER CONTRACTILITY

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology I1 Apr 2016MP60-08 CHRONIC PSYCHOLOGICAL STRESS INDUCES CHANGES IN BLADDER CONTRACTILITY Yunliang Gao, Huiyi Chang, Rong Zhang, and Larissa Rodriguez Yunliang GaoYunliang Gao More articles by this author , Huiyi ChangHuiyi Chang More articles by this author , Rong ZhangRong Zhang More articles by this author , and Larissa RodriguezLarissa Rodriguez More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.861AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Chronic psychological stress can impact lower urinary tract function. We aimed to evaluate if the voiding dysfunction and bladder hyperalgesia seen in the animals exposed to water avoidance stress (WAS) is partially due to changes in bladder contractility. METHODS Eight adult female Wistar-Kyoto rats were divided to 10-day WAS protocol (n=4) or handled control (n=4). On day 11, the urinary bladder was harvested, cut into strips, and kept in oxygenated Krebs solution. The remaining of the bladder and urethra were fixed in 10% formalin for histological studies. The contraction amplitude of each strip induced by KCl (80 µM) was regarded as 100% contraction amplitude for normalization. Carbachol, a non-selective cholinergic muscarinic receptor agonist, was given to examine the contractility (10-7 to 3×10-4 M). Atropine (10-6 M), a muscarinic receptor antagonist was given after carbachol to verify the response. Hematoxylin and eosin stain was used to assess tissue structure. RESULTS There were dose dependent responses to carbachol with maximum contractility observed at 10-5 M (Fig 1A). At maximum dose response, there was significantly higher contractility in the bladders of WAS rats than controls (27% higher). Atropine reversed the effect of carbachol on bladder contractility. The thickness and structure of the bladder wall and urethra did not show significantly changes between WAS rats and controls (Fig 1B-C). CONCLUSIONS Chronic psychological stress leads to changes in bladder contractility without changes in the structure of bladder and urethra. Expression or responsiveness of muscarinic receptors induced by stress play a role in the urinary frequency and voiding changes seen in animals exposed to WAS. Receptor characterization will be evaluated in future studies. These results have significant implications in human lower urinary pathologies that have been shown to be exacerbated by affected by stress such as urinary frequency, overactive bladders, and interstitial cystitis. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e796-e797 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Yunliang Gao More articles by this author Huiyi Chang More articles by this author Rong Zhang More articles by this author Larissa Rodriguez More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...