MP58-02 ABERRANT N-GLYCOSYLATION PROFILE OF SERUM IMMUNOGLOBULINS IS A DIAGNOSTIC BIOMARKER OF UROTHELIAL CARCINOMAS

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology II1 Apr 2018MP58-02 ABERRANT N-GLYCOSYLATION PROFILE OF SERUM IMMUNOGLOBULINS IS A DIAGNOSTIC BIOMARKER OF UROTHELIAL CARCINOMAS Toshikazu Tanaka, Tohru Yoneyama, Daisuke Noro, Yuta Kojima, Kengo Imanishi, Hayato Yamamoto, Yuki Tobisawa, Kazuyuki Mori, Atsushi Imai, Shingo Hatakeyama, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Masakazu Tanaka, Shin-Ichiro Nishimura, Shizuka Kurauchi, Ippei Takahashi, and Chikara Ohyama Toshikazu TanakaToshikazu Tanaka More articles by this author , Tohru YoneyamaTohru Yoneyama More articles by this author , Daisuke NoroDaisuke Noro More articles by this author , Yuta KojimaYuta Kojima More articles by this author , Kengo ImanishiKengo Imanishi More articles by this author , Hayato YamamotoHayato Yamamoto More articles by this author , Yuki TobisawaYuki Tobisawa More articles by this author , Kazuyuki MoriKazuyuki Mori More articles by this author , Atsushi ImaiAtsushi Imai More articles by this author , Shingo HatakeyamaShingo Hatakeyama More articles by this author , Takahiro YoneyamaTakahiro Yoneyama More articles by this author , Yasuhiro HashimotoYasuhiro Hashimoto More articles by this author , Takuya KoieTakuya Koie More articles by this author , Masakazu TanakaMasakazu Tanaka More articles by this author , Shin-Ichiro NishimuraShin-Ichiro Nishimura More articles by this author , Shizuka KurauchiShizuka Kurauchi More articles by this author , Ippei TakahashiIppei Takahashi More articles by this author , and Chikara OhyamaChikara Ohyama More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1834AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The clinical diagnosis of urothelial carcinoma (UC) relies on invasive methods in patients with hematuria. Although more sensitive and noninvasive screening methods are required, a specific serum biomarker for UC is lacking. To determine whether the aberrant N-glycosylated serum immunoglobulins (Igs) can be applied as a diagnostic marker of urothelial carcinoma (UC). METHODS Between 2009 and 2016, N-glycan levels on Igs were retrospectively examined in the sera of 60 upper urinary tract UC (UTUC), 177 UC of the bladder (UCB), 317 healthy volunteer (HV) controls and 96 prostate cancer (PC) patients by high-throughput N-glycomics. The diagnostic N-glycan score (dNGScore) for detection of UCs was established by multiplying five N-glycan levels on Igs by each function value that calculated by multivariable discriminant analysis. The diagnostic performance of dNGScore was evaluated by receiver operating characteristic curves and compare the diagnostic performance of classic urine cytology and hematuria. RESULTS Total 32 types of N-glycans were identified from Igs fraction of all samples. Of those 5 N-glycans (biantannary and core fucosylated bisecting GlcNAc type N-glycans) on Igs were selected as UC associated aberrant N-glycosylated Igs and we found asialo bisecting GlcNAc type N-glycan carrying Igs were accumulated in UC patients.The dNGScore established by combination of 5 N-glycans on Igs discriminated UC patients from HV and PC patient with 92.8% sensitivity, 97.2% specificity. The area under the curve (AUC) of dNGScore was 0.969 for UC detection that was much superior to that of urine cytology (AUC, 0.707) and hematuria (AUC, 0.892). Furthermore, dNGScore can detect hematuria and urine cytology negative patients. CONCLUSIONS dNGscore based on aberrant N-glycosylation signatures of Igs were found to be promising diagnostic biomarkers of UCs. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e773 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Toshikazu Tanaka More articles by this author Tohru Yoneyama More articles by this author Daisuke Noro More articles by this author Yuta Kojima More articles by this author Kengo Imanishi More articles by this author Hayato Yamamoto More articles by this author Yuki Tobisawa More articles by this author Kazuyuki Mori More articles by this author Atsushi Imai More articles by this author Shingo Hatakeyama More articles by this author Takahiro Yoneyama More articles by this author Yasuhiro Hashimoto More articles by this author Takuya Koie More articles by this author Masakazu Tanaka More articles by this author Shin-Ichiro Nishimura More articles by this author Shizuka Kurauchi More articles by this author Ippei Takahashi More articles by this author Chikara Ohyama More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...