MP56-03 EXPRESSION PROFILE OF EPITHELIAL-MESENCHYMAL TRANSITION MARKERS IN NON-MUSCLE INVASIVE UROTHELIAL CARCINOMA OF THE BLADDER: CORRELATION WITH INTRAVESICAL RECURRENCE FOLLOWING TRANSURETHRAL RESECTION

Abstract

You have accessJournal of UrologyBladder Cancer: Superficial1 Apr 2014MP56-03 EXPRESSION PROFILE OF EPITHELIAL-MESENCHYMAL TRANSITION MARKERS IN NON-MUSCLE INVASIVE UROTHELIAL CARCINOMA OF THE BLADDER: CORRELATION WITH INTRAVESICAL RECURRENCE FOLLOWING TRANSURETHRAL RESECTION Akira Miyazaki, Hideaki Miyake, Liu Bing, and Masato Fujisawa Akira MiyazakiAkira Miyazaki More articles by this author , Hideaki MiyakeHideaki Miyake More articles by this author , Liu BingLiu Bing More articles by this author , and Masato FujisawaMasato Fujisawa More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1571AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives Despite generally favorable prognosis in patients with non-muscle invasive urothelial carcinoma of the bladder (NMIUCB), intravesical recurrence following transurethral resection (TUR) has been reported in approximately 50% of patients with this disease. The objective of this study was to evaluate the expression of multiple molecular markers involved in epithelial-mesenchymal transition (EMT), a key process mediating the progression of a wide variety of malignant tumors, in NMIUCB to clarify the significance of these markers as predictors of intravesical recurrence in patients treated with TUR. Methods This study included a total of 161 patients with NMIUCB undergoing TUR at our institution. Expression levels of 13 EMT markers, including E-cadherin, N-cadherin, β-catenin, γ-catenin, fibronectin, MMP-2, MMP-9, Slug, Snail, Twist, vimentin ZEB1 and ZEB2, in TUR specimens obtained from these 161 patients were measured by immunohistochemical staining. These findings were analyzed according to several clinicopathological outcomes. Results Of these 13 proteins, expression levels of E-cadherin, N-cadherin, MMP-2, MMP-9 and TWIST significantly associated with several conventional predictive factors of intravesical recurrence. Univariate analysis identified expression level of E-cadherin, N-cadherin, MMP-2, MMP-9 and TWIST as significant predictors of intravesical recurrence as well, while tumor size, pathological T stage and concomitant carcinoma in situ (CIS) were also significant. Of these significant factors, expression levels of E-cadherin, MMP-9 and TWIST, tumor size and concomitant CIS appeared to be independently related to intravesical recurrence by multivariate analysis. Furthermore, there were significant differences in recurrence-free survival according to positive numbers of these three independent risk factors (i.e., negative vs positive for 1 or 2 factor vs positive for 3 or more factors). Conclusions Consideration of expression levels of EMT-associated markers in TUR specimens, in addition to conventional prognostic parameters, would contribute to accurate prediction of intravesical recurrence following TUR for NMIUCB. Moreover, combined evaluation of expression levels of E-cadherin, MMP-9 and TWIST, tumor size and concomitant CIS would be particularly useful for further refinement of the system in predicting recurrence of NMIUCB following TUR. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e564 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Akira Miyazaki More articles by this author Hideaki Miyake More articles by this author Liu Bing More articles by this author Masato Fujisawa More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...