MP54-10 UTILITY OF A URINE-BASED DNA METHYLATION TEST FOR SURVEILLANCE IN NON-MUSCLE INVASIVE BLADDER CANCER: A PILOT STUDY

Abstract

You have accessJournal of UrologyCME1 May 2022MP54-10 UTILITY OF A URINE-BASED DNA METHYLATION TEST FOR SURVEILLANCE IN NON-MUSCLE INVASIVE BLADDER CANCER: A PILOT STUDY Seyedeh Sanam Ladi Seyedian, Alireza Ghoreifi, Sidney Roberts, Farshad Sheybaee Moghadam, Hamed Ahmadi, Saum Ghodoussipour, Muhannad Alsyouf, Paolo Piatti, Benjamin Jara, Lucy Sanossian, Sumeet Bhanvadia, Anne Schuckman, Hooman Djaladat, Gangning Liang, and Siamak Daneshmand Seyedeh Sanam Ladi SeyedianSeyedeh Sanam Ladi Seyedian More articles by this author , Alireza GhoreifiAlireza Ghoreifi More articles by this author , Sidney RobertsSidney Roberts More articles by this author , Farshad Sheybaee MoghadamFarshad Sheybaee Moghadam More articles by this author , Hamed AhmadiHamed Ahmadi More articles by this author , Saum GhodoussipourSaum Ghodoussipour More articles by this author , Muhannad AlsyoufMuhannad Alsyouf More articles by this author , Paolo PiattiPaolo Piatti More articles by this author , Benjamin JaraBenjamin Jara More articles by this author , Lucy SanossianLucy Sanossian More articles by this author , Sumeet BhanvadiaSumeet Bhanvadia More articles by this author , Anne SchuckmanAnne Schuckman More articles by this author , Hooman DjaladatHooman Djaladat More articles by this author , Gangning LiangGangning Liang More articles by this author , and Siamak DaneshmandSiamak Daneshmand More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002633.10AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Currently available urine-based tests used for surveillance of non-muscle invasive bladder cancer (NMIBC) are suboptimal. Here, we explore the feasibility of a newly developed urine-based DNA methylation test for detection of recurrence in NMIBC. METHODS: Patients who had evidence of bladder cancer recurrence on surveillance cystoscopy or were referred to our center for blue light transurethral resection of bladder tumor (TURBT) between October 2019 and August 2021 were enrolled. Urine samples were collected before and two months after TURBT and then at each surveillance cystoscopy afterwards. Samples were analyzed with Bladder CARE, a urine-based assay that measures methylation levels of 3 bladder cancer specific biomarkers (TRNA-Cys, SIM2, and NKX1-1) and two internal control loci using methylation-sensitive restriction enzymes coupled with qPCR. Results are reported as Bladder CARE Index (BCI) score and categorized as “positive” (BCI >5) or “negative” (BCI ≤5). Association between change in BCI score and category with TURBT pathology and recurrence was assessed. RESULTS: A total of 190 patients with NMIBC were included (median age of 73, 77% male). Of patients under surveillance during the study period, 40/147 (27%) had recurrence, in all but four the BCI became positive prior to or at the time of recurrence. BCI was able to predict the recurrence within a median of 7 months prior. Post-TURBT samples were available in 39/95 (41%) patients. Patients with a positive BCI two months following the TURBT had significantly lower recurrence free survival at 2 years (P= 0.001, Fig. 1). The Receiver Operating Characteristic (ROC) curve demonstrated the sensitivity, specificity, positive predictive values, and negative predictive value of 93%, 65%, 73.5%, and 89.5%, respectively (AUC=0.867, Fig. 2). The false positive and negative rate of Bladder CARE was 35% and 7.3%, respectively. Cytology was only positive or atypical in 27% of the recurrences. CONCLUSIONS: Our preliminary findings suggest a potential utility for this urine-based methylation test in early detection of recurrence in NMIBC. Larger sample size with longer follow-up is required to validate these outcomes. Source of Funding: Zymo Research Corp, Pangea Laboratory © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e930 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Seyedeh Sanam Ladi Seyedian More articles by this author Alireza Ghoreifi More articles by this author Sidney Roberts More articles by this author Farshad Sheybaee Moghadam More articles by this author Hamed Ahmadi More articles by this author Saum Ghodoussipour More articles by this author Muhannad Alsyouf More articles by this author Paolo Piatti More articles by this author Benjamin Jara More articles by this author Lucy Sanossian More articles by this author Sumeet Bhanvadia More articles by this author Anne Schuckman More articles by this author Hooman Djaladat More articles by this author Gangning Liang More articles by this author Siamak Daneshmand More articles by this author Expand All Advertisement PDF DownloadLoading ...