MP52-17 EXPRESSION AND DISTRIBUTION OF THE TRANSIENT RECEPTOR POTENTIAL CATIONIC CHANNEL A1 (TRPA1) IN HUMAN PENILE ERECTILE TISSUE AND THE SEMINAL VESICLES

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research I1 Apr 2015MP52-17 EXPRESSION AND DISTRIBUTION OF THE TRANSIENT RECEPTOR POTENTIAL CATIONIC CHANNEL A1 (TRPA1) IN HUMAN PENILE ERECTILE TISSUE AND THE SEMINAL VESICLES Stefan Ückert, Andreas Bannowsky, Markus Kuczyk, and Petter Hedlund Stefan ÜckertStefan Ückert More articles by this author , Andreas BannowskyAndreas Bannowsky More articles by this author , Markus KuczykMarkus Kuczyk More articles by this author , and Petter HedlundPetter Hedlund More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1734AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The transient receptor potential cationic channel A1 (TRPA1) has been suggested to be involved in mechano-afferent/efferent signaling in the bladder, prostate, and urethra. Up until today, no study has addressed the expression of this receptor in male genital and reproductive tissues. Thus, it was the aim of the present study to evaluate in human penile erectile tissue (corpus cavernosum penis) and the seminal vesicles (SV) by means of molecular biology and immunohistochemistry the expression and localization of TRPA/TRPA1. METHODS Human penile erectile tissue was obtained from 5 subjects who underwent reassignment surgery. SV tissue was excised from 5 male patients during pelvic surgery due to malignancies of the prostate or urinary bladder. Using immunohistochemical methods (double-labelling technique, laser fluorescence microscopy), the distribution of TRPA1 in relation to neuronal nitric oxide synthase (nNOS), the neuropeptide vasoactive intestinal polypeptide (VIP), and vesicular acetylcholine transporter protein (VAChT) was examined in sections of the CC, while, in sections of the SV, the distribution of TRPA1 was investigated in relation to eNOS, nNOS, VIP and CGRP. RESULTS In the CC, immunoreactivity for TRPA1 was located in nerves transversing the cavernous sinusoidal space. These nerves also displayed the expression of VAChT. Signals specific for TRPA1 were also observed in meshworks of nerve fibers running alongside the walls of cavernous arteries. Varicose nerves containing nNOS or VIP were not immunoreactive for TRPA1. In the SV, TRPA1 was seen expressed in nerves running through the smooth muscle portion. Here, the protein was in part colocalized with nNOS and CGRP, whereas no colocalization with VIP was observed. Dot-like signals specific for TRPA1 were observed in the cytoplasm of epithelial cells lining the lacunar lumen of glandular spaces. The epithelial layer also presented staining for eNOS. Both the vascular and non-vascular smooth muscle of CC and SV appeared free of immunosignals related to TRPA1. CONCLUSIONS The distribution of TRPA1 and VAChT receptors in penile erectile tissue suggests a role for TRPA1 in the mechanism of cholinergic signaling in the human penis while, in the SV, TRPA1 might be involved in the control of secretory function (mediated by cyclic GMP). © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e633 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Stefan Ückert More articles by this author Andreas Bannowsky More articles by this author Markus Kuczyk More articles by this author Petter Hedlund More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...