MP52-03 THE PERICYTES AS A CELLULAR REGULATOR OF PENILE ERECTION AND A NOVEL THERAPEUTIC TARGET FOR ERECTILE DYSFUNCTION

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research I1 Apr 2015MP52-03 THE PERICYTES AS A CELLULAR REGULATOR OF PENILE ERECTION AND A NOVEL THERAPEUTIC TARGET FOR ERECTILE DYSFUNCTION Guo Nan Yin, Nando Dulal Das, Min Ji Choi, Kang-Moon Song, Mi-Hye Kwon, Jiyeon Ock, Anita Limanjaya, Kalyan Ghatak, Woo Jean Kim, Soo-Hwan Park, Jae Seog Hyun, Ji-Kan Ryu, and Jun-Kyu Suh Guo Nan YinGuo Nan Yin More articles by this author , Nando Dulal DasNando Dulal Das More articles by this author , Min Ji ChoiMin Ji Choi More articles by this author , Kang-Moon SongKang-Moon Song More articles by this author , Mi-Hye KwonMi-Hye Kwon More articles by this author , Jiyeon OckJiyeon Ock More articles by this author , Anita LimanjayaAnita Limanjaya More articles by this author , Kalyan GhatakKalyan Ghatak More articles by this author , Woo Jean KimWoo Jean Kim More articles by this author , Soo-Hwan ParkSoo-Hwan Park More articles by this author , Jae Seog HyunJae Seog Hyun More articles by this author , Ji-Kan RyuJi-Kan Ryu More articles by this author , and Jun-Kyu SuhJun-Kyu Suh More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1720AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Pericytes are known to play critical roles in vascular development and homeostasis. The aim of this study was to examine the differential expression of pericytes in the penis and their functional roles in penile erection. METHODS The diabetic mice were treated with hepatocyte growth factor (HGF) protein to enhance pericyte function and normal mice received anti-platelet-derived growth factor receptor-beta (PDGFR-β) blocking antibody to inhibit pericyte function. Erectile function was measured by electrical stimulation of the cavernous nerve. Penile tissue was obtained from diabetic mice and from patients with psychogenic erectile dysfunction. The differential distribution of pericytes in mouse and human penis was determined. Data were analyzed by using Mann–Whitney U-tests or one-way analysis of variance. RESULTS Pericytes were abundantly distributed in microvessels of the subtunical area and dorsal nerve bundle of mice, followed by dorsal vein and cavernous sinusoids. We further confirmed the presence of pericytes in human corpus cavernosum tissue and successfully isolated pericytes from mouse penis. Inhibition of pericyte function with anti-PDGFR-β blocking antibody induced deterioration of erectile function in normal mice. In contrast, HGF protein restored cavernous pericyte content in diabetic mice, and significantly decreased cavernous vascular permeability in diabetic mice in vivo and in pericytes-endothelial cell co-culture system in vitro, which induced significant recovery of erectile function. CONCLUSIONS Pericyte gain- and loss-of-function studies suggest that the functional and structural integrity of pericytes are critical for maintaining penile erection. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e628-e629 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Guo Nan Yin More articles by this author Nando Dulal Das More articles by this author Min Ji Choi More articles by this author Kang-Moon Song More articles by this author Mi-Hye Kwon More articles by this author Jiyeon Ock More articles by this author Anita Limanjaya More articles by this author Kalyan Ghatak More articles by this author Woo Jean Kim More articles by this author Soo-Hwan Park More articles by this author Jae Seog Hyun More articles by this author Ji-Kan Ryu More articles by this author Jun-Kyu Suh More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...