MP51-18 DIFFERENTIAL EXPRESSION PROFILING OF LNCRNA IN PRIMARY PROSTATE CANCER BASED ON BLACK AND WHITE RACE

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research & Pathophysiology II (MP51)1 Apr 2020MP51-18 DIFFERENTIAL EXPRESSION PROFILING OF LNCRNA IN PRIMARY PROSTATE CANCER BASED ON BLACK AND WHITE RACE Adam Weiner*, Eddie-Luidy Imada, Edward Schaeffer, and Luigi Marchionni Adam Weiner*Adam Weiner* More articles by this author , Eddie-Luidy ImadaEddie-Luidy Imada More articles by this author , Edward SchaefferEdward Schaeffer More articles by this author , and Luigi MarchionniLuigi Marchionni More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000913.018AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Black men are more likely to be diagnosed with and die from prostate cancer (PC) compared to white men. Long, non-coding RNAs (lncRNAs) govern gene expression with increasingly noted relevance in cancer biology. To better understand biological factors driving racial disparities in PC outcomes, we performed a differential expression and functional analysis of lncRNAs based on race. METHODS: We utilized the FANTOM-CAT/recount2 atlas, a powerful resource combining data on expression profiles for over 100,000 genes and the functional characterization of human coding and non-coding transcriptomes. From data on primary PC in 43 black men and 270 white men within the Cancer Genome Atlas, differential gene expression analyses were performed with negative binomial generalized linear models and quasi-likelihood F-test. Genes with FDR <= 0.05 were considered differentially expressed. ChIPBase data were leveraged to classify lncRNAs as androgen responsive if an androgen receptor regulatory domain was within 10 kb up- or 1 kb down-stream of the lncRNAs’ transcription start site. RESULTS: In total, 182 lncRNA were differentially expressed in PC from black men (Figure). Of these, 112 were annotated as gene promoters and/or enhancers. Based on ChIPBase data, 8 of these annotated lncRNAs were classified as androgen responsive, all of which were expressed more than 10-fold in black men (RP11-1055B8.4, RP11-131N11.4, RP11-131N11.4, RP11-131N11.4, RP11-359J6.1, RP11-359J6.1, RP11-536I6.2, and RP11-536I6.2). CONCLUSIONS: We provide a comprehensive differential expression and functional analysis of lncRNAs in primary PC based on race. Most of these lncRNAs were annotated as gene promoters and/or enhancers and several are potentially androgen responsive. These results suggest a unique pathway for PC progression in black men regulated by lncRNA, each of which represents potential biomarkers and targets for therapeutics. Source of Funding: National Institutes of Health grant 5U01CA196390 and the Prostate Cancer Foundation (EMS) as well as the 2019 Urology Care Foundation Residency Research Award Program and the Russell Scott, Jr., MD Urology Research Fund (ABW). © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e770-e771 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Adam Weiner* More articles by this author Eddie-Luidy Imada More articles by this author Edward Schaeffer More articles by this author Luigi Marchionni More articles by this author Expand All Advertisement PDF downloadLoading ...