MP47-09 HKLK1 IMPROVES THE ERECTILE FUNCTION IN AGED RATS BY ENHANCING ENOS AND INOS EXPRESSON AND ANTI-TISSUE FIBROSIS EFFECT

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research II1 Apr 2014MP47-09 HKLK1 IMPROVES THE ERECTILE FUNCTION IN AGED RATS BY ENHANCING ENOS AND INOS EXPRESSON AND ANTI-TISSUE FIBROSIS EFFECT Yang Luan, Tao Wang, Li Zhuan, Yan Zhang, Ya-Jun Ruan, Shao-Gang Wang, Ji-Hong Liu, and Zhang-Qun Ye Yang LuanYang Luan More articles by this author , Tao WangTao Wang More articles by this author , Li ZhuanLi Zhuan More articles by this author , Yan ZhangYan Zhang More articles by this author , Ya-Jun RuanYa-Jun Ruan More articles by this author , Shao-Gang WangShao-Gang Wang More articles by this author , Ji-Hong LiuJi-Hong Liu More articles by this author , and Zhang-Qun YeZhang-Qun Ye More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1462AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives Age is the most common risk factor for erectile dysfunction (ED). Human tissue kallikrein1 (hKLK1), as a pleiotropic agent, plays an important role in vasodilation, endothelial cell protection, anti-tissue fibrosis, etc. We aim to explore the effect and preliminarily test the mechanisms of hKLK1 on age-related erectile dysfunction with transgenic rats harboring hKLK1gene (TGR(hKLK1). Methods Four groups of adult male Sprague-Dawley rats were involved in this study: 1) young wild type rats (yWTR, 4 months, n = 10) as control group; 2) young TGR(hKLK1) (yTGR, 4 months, n = 10) group; 3) aged wild type rats (aWTR, 27 months, n = 10) group; 4) aged TGR (hKLK1) (aTGR, 27 months, n = 26) group. Extraction of genome DNA followed by conventional polymerase chain reaction (PCR) and real-time PCR after reverse transcription-PCR of tissue RNA were performed to identify the existence of hKLK1gene. The ratio of peak intracavernous pressure (ICP) to systemic mean arterial pressure (MAP) were measured at various voltages of electrical stimulation to the cavernous nerve (CN) to evaluate the erectile function of rats in each group. The eNOS and iNOS expression in penis were detected by real-time PCR. The ratios of smooth muscle to collagen and apoptosis within the corporal tissue were analyzed by masson's trichrome staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, respectively. Results All TGR (hKLK1) carried hKLK1gene while wild type rats negtively contained it. No conspicuous diversity was found between yWTR and yTGR groups at electrostimulation to CN, real-time PCR, masson's trichrome staining and TUNEL assays. The ICP/MAP ratio of aWTR group was decreased compared with the control group (P<0.05), but the erectile function was greatly improved in aTGR group relative to aWTR group (P<0.05, respectively) and reached the level of control group at all kinds of voltages of 2.5v, 5.0v and 7.5v. The expression of mRNA for eNOS in aTGR was markedly higher than that in aWTR (P<0.05), although it was still lower than yWTR (P<0.01). Moreover, the iNOS mRNA expression in aTGR was obviously increased than that in aWTR and yWTR (P<0.05 of both). The reduction of the smooth muscle/collagen in aWTR, relative to yWTR, was significantly attenuated in aTGR (P<0.01). Meanwhile, the apoptosis data showed the same tendency towards the results of masson's trichrome staining (P<0.001). Conclusions Our results suggest that hKLK1 may improve ED in aged rats via promoting eNOS and iNOS expression and anti-tissue fibosis as well as anti-apoptosis effect. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e521 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yang Luan More articles by this author Tao Wang More articles by this author Li Zhuan More articles by this author Yan Zhang More articles by this author Ya-Jun Ruan More articles by this author Shao-Gang Wang More articles by this author Ji-Hong Liu More articles by this author Zhang-Qun Ye More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...