MP47-08 INTRATUNICAL INJECTION OF HUMAN ADIPOSE TISSUE–DERIVED STEM PARTIALLY REVERTS FIBROSIS AND RESTORES COLLAGEN III/I RATIO IN A RAT MODEL OF CHRONIC PEYRONIE’S DISEASE

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research II1 Apr 2014MP47-08 INTRATUNICAL INJECTION OF HUMAN ADIPOSE TISSUE–DERIVED STEM PARTIALLY REVERTS FIBROSIS AND RESTORES COLLAGEN III/I RATIO IN A RAT MODEL OF CHRONIC PEYRONIE’S DISEASE Fabio Castiglione, Maarten Al, Ettore Di trapani, Alessandro Nini, Trinity TJ. Bivalacqua, Johanna L. Hannan, Giorgia Colciago, Andrea Salonia, Fabio Benigni, Francesco Montorsi, and Petter Hedlund Fabio CastiglioneFabio Castiglione More articles by this author , Maarten AlMaarten Al More articles by this author , Ettore Di trapaniEttore Di trapani More articles by this author , Alessandro NiniAlessandro Nini More articles by this author , Trinity TJ. BivalacquaTrinity TJ. Bivalacqua More articles by this author , Johanna L. HannanJohanna L. Hannan More articles by this author , Giorgia ColciagoGiorgia Colciago More articles by this author , Andrea SaloniaAndrea Salonia More articles by this author , Fabio BenigniFabio Benigni More articles by this author , Francesco MontorsiFrancesco Montorsi More articles by this author , and Petter HedlundPetter Hedlund More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1461AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives Peyronie's disease (PD) is a connective tissue disorder of the tunica albuginea (TA). The aim of this study wasto test the effects of a local injection of human adipose tissue-derived stem cells (hADSCs) in the chronic phase of a rat model of PD. Methods A total of 21 male 12-wk-old Sprague-Dawley rats were divided in three groups and underwent injection of vehicle (sham), 0.5-ìg transforming growth factor (TGF-â1) in a 50-ìl vehicle (in either a PD or a PD plus hADSC group) in the dorsal aspect of the TA. The sham and PD groups were treated 1 month after vehicle or TGF-â1 injection with intratunical treatment of vehicle, and the ADSC group received 1 million of hADSCs in the 50-ìl vehicle. 10 weeks after treatment, following euthanasia, penises were harvested for histology and Western blot analysis (WB).Quantificationof signals on the PVDF membrane was performed usingKodak Image Station 440 and SigmaScan Pro 5.0 software. Comparisons were performed with an ANOVA test. Values are expressed mean± standard error of the mean (SEM) Results Byimmunofluorescence,PD animals had developed areas of fibrosis of their peniseswith up-regulation of collagen III compared to sham animals and the hADSCs group. At WB analysis,collagen III expression was higher in PD (mean: 1.35±0.40) than in sham and hADSCs groups (0.24±0.129 and 0.39±0.07 respectively; p< 0.05). Elastin was less expressed in sham animals (0.11±0.03) compared to hADSCs (0.24±0.03) and PD groups (0.25±0.04; p<0.05). No differenceswere noted in collagen I expressionbetween the three groups.Interestingly, the collagen III/I ratio was higher in PD (4.6±0.92) group compared to sham (0.66±0.18) and hADSCs groups (0.86±0.06) (p<0.05). Conclusions Injection of ADSCs into the TA partially reverted the fibrosis and restored the Collagen III/I ratio to normal values in a rat model of chronic PD. hADSCs treatment was not able to reduce elastosis. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e521 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Fabio Castiglione More articles by this author Maarten Al More articles by this author Ettore Di trapani More articles by this author Alessandro Nini More articles by this author Trinity TJ. Bivalacqua More articles by this author Johanna L. Hannan More articles by this author Giorgia Colciago More articles by this author Andrea Salonia More articles by this author Fabio Benigni More articles by this author Francesco Montorsi More articles by this author Petter Hedlund More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...