MP47-08 THE METHYLATION OF SOX7 AND ITS FUNCTION AS A TUMOR SUPPRESSOR IN RENAL CELL CANCER

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research II1 Apr 2015MP47-08 THE METHYLATION OF SOX7 AND ITS FUNCTION AS A TUMOR SUPPRESSOR IN RENAL CELL CANCER Lu Wang, Qian Zhang, Lian Zhang, Tingxiu Xiang, Yu Fan, Ben Xu, Qian Tao, and Jie Jin Lu WangLu Wang More articles by this author , Qian ZhangQian Zhang More articles by this author , Lian ZhangLian Zhang More articles by this author , Tingxiu XiangTingxiu Xiang More articles by this author , Yu FanYu Fan More articles by this author , Ben XuBen Xu More articles by this author , Qian TaoQian Tao More articles by this author , and Jie JinJie Jin More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1528AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Sox7, as a member of SRY (sex determining region Y) transcription factor family, plays an important role in the development and tumororiegnesis of various kinds of tumors. However, its function in renal cell cancer(RCC) has not been reported. Thus we studied the regulation of sox7 expression and its related function in RCC. METHODS We detected the expression of sox7 in RCC cell lines and primary RCC tissues by semi-quantitative RT-PCR, quantitative RT-PCR and western-blot.we also used Methylation-specific PCR and Bisulfite Genomic Sequencing to observe the methylation status of sox7 in cell lines, primary RCC tissues and RCC surgical margin tissues. In order to further verify the tumor suppressive effects of sox7, cell functional experiments including colony formation,wound-healing assay and transwell were used with 786-O and A498 cell lines.we also measured the mRNA levels of survivin and cyclinD1 to examine the relationship between WNT signaling pathway and sox7. RESULTS The expression level of sox7 was significantly down-regulated in RCC cell lines (7/9) and primary RCC tissues by aberrant promoter methylation, compared with nontumorigenic cells and adjacent normal tissues. By analyzing the correlation between clinical features and sox7 methylation status. Sox7 methylation status was detected in 21% of kidney tumour samples, whereas only rarely methylated in surgical margin tissues. The expression level of sox7 increased after pharmacologic demethylation treatment. Ectopic expression of sox7 inhibits cell proliferation through inducing G0/G1 cell cycle arrest,overexpression of sox7 can also suppress migration,and invasion of renal cancer cells in vitro. Moreover, mRNA expression level of Wnt signaling pathway targeted cyclinD1 and survivin were reduced upon sox7 overexpression in 786-o and A498 RCC cells. CONCLUSIONS In conclusion,our results first imply that sox7 functions as a tumor suppressor in RCC and is frequently down-regulated,which was mainly due to the DNA promoter hypermethylation. The methylation of sox7 is frequently occured in RCC patients of early stage. Therefore sox7 may be regarded as a biomarker for diagnosis of RCC. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e554-e555 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Lu Wang More articles by this author Qian Zhang More articles by this author Lian Zhang More articles by this author Tingxiu Xiang More articles by this author Yu Fan More articles by this author Ben Xu More articles by this author Qian Tao More articles by this author Jie Jin More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...