Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology I1 Apr 2017MP44-04 SIGNIFICANCE OF SERUM N-GLYCAN PROFILING AS A DIAGNOSTIC BIOMARKER IN UROTHELIAL CARCINOMA Masaaki Oikawa, Shingo Hatakeyama, Tohru Yoneyma, Yuki Tobisawa, Takuma Narita, Hayato Yamamoto, Yasuhiro Hashimoto, Takuya Koie, Shintaro Narita, Atsushi Sasaki, Norihiko Tsuchiya, Tomonori Habuchi, Ippei Takahashi, Shigeyuki Nakaji, and Chikara Ohyama Masaaki OikawaMasaaki Oikawa More articles by this author , Shingo HatakeyamaShingo Hatakeyama More articles by this author , Tohru YoneymaTohru Yoneyma More articles by this author , Yuki TobisawaYuki Tobisawa More articles by this author , Takuma NaritaTakuma Narita More articles by this author , Hayato YamamotoHayato Yamamoto More articles by this author , Yasuhiro HashimotoYasuhiro Hashimoto More articles by this author , Takuya KoieTakuya Koie More articles by this author , Shintaro NaritaShintaro Narita More articles by this author , Atsushi SasakiAtsushi Sasaki More articles by this author , Norihiko TsuchiyaNorihiko Tsuchiya More articles by this author , Tomonori HabuchiTomonori Habuchi More articles by this author , Ippei TakahashiIppei Takahashi More articles by this author , Shigeyuki NakajiShigeyuki Nakaji More articles by this author , and Chikara OhyamaChikara Ohyama More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.1335AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The clinical diagnosis of urothelial carcinoma (UC) relies on invasive methods in patients with hematuria. Although more sensitive and noninvasive screening methods are required, a specific serum biomarker for UC is lacking.To examine whether the serum glycan-based biomarkers can be applied to detecting UC. METHODS Between April 1994 and June 2016, serum N-glycan concentrations were retrospectively measured in 212 patients with UC before treatment (UC group) and 212 pair-matched controls (VLT group) using glycoblotting and mass spectrometry.N-glycan levels were compared between the groups using receiver operating characteristic (ROC) curves to select candidate N-glycans. We created an N-glycan score based on the combination of candidate N-glycans. The specificity and sensitivity of the candidate N-glycan score were evaluated using ROC curves. RESULTS The N-glycan score was calculated using six N-glycans (m/z 1566, 1687, 1769, 1871, 2011, 2337) that were significantly associated with UC. The median N-glycan score was significantly higher in the UC group than in the VLT group (5.0 vs. 1.0, P < 0.001). The predictive value of the N-glycan score for UC detection was significant, with a sensitivity, specificity, and area under the curve of 93%, 81%, and 0.95%, respectively. CONCLUSIONS Serum N-glycan content has the potential to be a specific and sensitive novel serum biomarker that may improve the accuracy of detection for UC and reduce unnecessary invasive screening. Validation of this test in a large-scale prospective study is needed. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e564-e565 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Masaaki Oikawa More articles by this author Shingo Hatakeyama More articles by this author Tohru Yoneyma More articles by this author Yuki Tobisawa More articles by this author Takuma Narita More articles by this author Hayato Yamamoto More articles by this author Yasuhiro Hashimoto More articles by this author Takuya Koie More articles by this author Shintaro Narita More articles by this author Atsushi Sasaki More articles by this author Norihiko Tsuchiya More articles by this author Tomonori Habuchi More articles by this author Ippei Takahashi More articles by this author Shigeyuki Nakaji More articles by this author Chikara Ohyama More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

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