MP43-09 THE EFFECT OF PNTX2-6 PROTEIN FROM PHONEUTRIA NIGRIVENTER SPIDER TOXIN ON IMPROVEMENT OF ERECTILE DYSFUNCTION IN A RAT MODEL OF CAVERNOUS NERVE INJURY.

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research I1 Apr 2014MP43-09 THE EFFECT OF PNTX2-6 PROTEIN FROM PHONEUTRIA NIGRIVENTER SPIDER TOXIN ON IMPROVEMENT OF ERECTILE DYSFUNCTION IN A RAT MODEL OF CAVERNOUS NERVE INJURY. Yong Sun Choi, Ae Ryang Jung, Sung-Hoo Hong, Sae Woong Kim, Tae-Kon Hwang, and Ji Youl Lee Yong Sun ChoiYong Sun Choi More articles by this author , Ae Ryang JungAe Ryang Jung More articles by this author , Sung-Hoo HongSung-Hoo Hong More articles by this author , Sae Woong KimSae Woong Kim More articles by this author , Tae-Kon HwangTae-Kon Hwang More articles by this author , and Ji Youl LeeJi Youl Lee More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1166AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives Patients bitten by Phoneutria nigriventer show diverse symptoms involving priapism. This spider venom has been found to augment erectile function by increasing nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) levels. PnTx2-6 of Phoneutria nigriventer venom may be utilized for pharmacological therapy for penile erection.This study was conducted to explore the therapeutic efficacy of subcutaneous PnTx2-6 injection in a rat model of bilateral cavernous nerve crush injury (BCNI) through increased nitric oxide and cyclic guanosine monophosphate (cGMP) levels. Methods Rats were randomly assigned into six groups: Normal group, BCNI group, PBS group (BCNI group with PBS injection), N/C group (BCNI group with Negative control injection), W/T group (BCNI group with wild type recombinant Baculovirus injection), and SEAN group (BCNI group with recombinant Baculovirus containing PnTx2-6 gene injection). Each rat was injected with respective group-assigned agents into the subcutaneous corpus cavernosum once every three days. Four weeks after the operation, we demonstrated ratios of intracavernous pressure (ICP) to mean arterial pressure (MAP). The contents of smooth muscle and collagen were evaluated by Masson¡¯s trichrome staining. The expression of neuronal nitric oxide synthase (nNOS), alpha smooth muscle (¥á-SMA), and endothelial nitric oxide synthase (eNOS) were evaluated by immunohistochemistry. The cyclic guanosine monophosphate (cGMP) level in the corpus cavernosum was determined by cGMP assay. Results The ratio of ICP/MAP was significantly higher in the SEAN group compared to the other experimental groups. The SEAN group also had increased smooth muscle/collagen ratio. The expression of nNOS, ¥á-SMA in SEAN groups was significantly increased, compared with others groups. However, eNOS levels were not significantly different across the groups. The cGMP level was increased in the SEAN group. Conclusions PnTx2-6 improved erectile function and prevented muscle atrophy in a rat model of BCNI via increased NO/cGMP. Therefore, PnTx2-6 may be utilized as a pharmacological therapy for post-prostatectomy ED. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e480 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yong Sun Choi More articles by this author Ae Ryang Jung More articles by this author Sung-Hoo Hong More articles by this author Sae Woong Kim More articles by this author Tae-Kon Hwang More articles by this author Ji Youl Lee More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...