MP43-11 EIGHT-YEAR FERTILITY PRESERVATION PROGRAM AT ATRIUM HEALTH WAKE FOREST BAPTIST FOCUSING ON SPERMATOGONIAL STEM CELL AND ROUND SPERMATID TECHNOLOGIES.

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP43-11 EIGHT-YEAR FERTILITY PRESERVATION PROGRAM AT ATRIUM HEALTH WAKE FOREST BAPTIST FOCUSING ON SPERMATOGONIAL STEM CELL AND ROUND SPERMATID TECHNOLOGIES. Adam Cohen, Janmejay Hingu, Nima Pourhabibi Zarandi, Guillermo Galdon, Omar Abdelaal, Banafsheh Nickmehr, Kimberly Stogner-Underwood, Stanley Kogan, Steve Hodges, Stuart Howards, Thomas McLean, Anthony Atala, and Hooman Sadri-Ardekani Adam CohenAdam Cohen More articles by this author , Janmejay HinguJanmejay Hingu More articles by this author , Nima Pourhabibi ZarandiNima Pourhabibi Zarandi More articles by this author , Guillermo GaldonGuillermo Galdon More articles by this author , Omar AbdelaalOmar Abdelaal More articles by this author , Banafsheh NickmehrBanafsheh Nickmehr More articles by this author , Kimberly Stogner-UnderwoodKimberly Stogner-Underwood More articles by this author , Stanley KoganStanley Kogan More articles by this author , Steve HodgesSteve Hodges More articles by this author , Stuart HowardsStuart Howards More articles by this author , Thomas McLeanThomas McLean More articles by this author , Anthony AtalaAnthony Atala More articles by this author , and Hooman Sadri-ArdekaniHooman Sadri-Ardekani More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003289.11AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Male infertility has been associated with many disease processes, including chemotherapy and radiotherapy for cancer, undescended testis (UDT), and genetic conditions such as Klinefelter Syndrome (KS). Preservation of human spermatogonial stem cells (SSC) may be a suitable option for young male patients at risk of losing fertility. With optimal cryopreservation, cell viability can be retained to reestablish spermatogenesis in the future through in vitro germ cell differentiation or round spermatid injection. METHODS: Urology and hematology-oncology patients at Atrium Health Wake Forest Baptist with malignancy, UDT, or KS were approached for consent and assent (if ≥seven years old) for the fertility preservation program. If participation was accepted, patients were given the option to undergo a fertility-preserving testicular biopsy, preferably during general anesthesia for other procedures such as Port-a-Cath placement, orchidopexy, or lymph node biopsy. They were also allowed to enroll as a registry participant (non-biopsy control). Histological and microbiological analyses were performed on biopsies before being cryopreserved. Further data were obtained retrospectively via chart review. RESULTS: Over eight years of banking, a total of 202 patients were approached for the study, with 125 (61.8%) undergoing a biopsy, 39 (19.3%) enrolling as non-biopsy control, and 38 (18.8%) declining participation. The mean age at which biopsy was performed across all diagnoses was 8.39 years (range 0.61 to 37.39). Of the 125 biopsy patients, 73% opted to donate a portion (up to 20%) of the tissue for basic research with no direct benefit to the donor. Of the biopsied patients, 76 (60.8%) were performed for cancer, 38 (30.4%) for unilateral or bilateral UDT, and 11 (8.8%) for KS. Based on PRM1 immunofluorescence, 48.3% of specimens had round spermatids. Short-term complications for testicular biopsy, primarily pain and swelling, were typically mild to moderate. CONCLUSIONS: A multidisciplinary approach can accommodate patients at risk for reduced future fertility due to various pathologies. The preservation of testicular tissue at present can aid in future fertility restoration through techniques such as in vitro SSC differentiation or round cell spermatid injection (ROSI). Source of Funding: N/A © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e605 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Adam Cohen More articles by this author Janmejay Hingu More articles by this author Nima Pourhabibi Zarandi More articles by this author Guillermo Galdon More articles by this author Omar Abdelaal More articles by this author Banafsheh Nickmehr More articles by this author Kimberly Stogner-Underwood More articles by this author Stanley Kogan More articles by this author Steve Hodges More articles by this author Stuart Howards More articles by this author Thomas McLean More articles by this author Anthony Atala More articles by this author Hooman Sadri-Ardekani More articles by this author Expand All Advertisement PDF downloadLoading ...