MP42-19 BILAYERED AUTOLOGOUS ADIPOSE-DERIVED MESENCHYMAL CELL-GELATIN SHEETS RECONSTRUCT URETERAL TISSUES IN RABBITS

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP42-19 BILAYERED AUTOLOGOUS ADIPOSE-DERIVED MESENCHYMAL CELL-GELATIN SHEETS RECONSTRUCT URETERAL TISSUES IN RABBITS Noriyuki Ogawa, Tetsuya Imamura, Tomonori Minagawa, Teruyuki Ogawa, and Osamu Ishizuka Noriyuki OgawaNoriyuki Ogawa More articles by this author , Tetsuya ImamuraTetsuya Imamura More articles by this author , Tomonori MinagawaTomonori Minagawa More articles by this author , Teruyuki OgawaTeruyuki Ogawa More articles by this author , and Osamu IshizukaOsamu Ishizuka More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003280.19AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Treatments of ureteral defects or strictures due to disease or trauma require reconstruction by urological surgery. However, those surgeries have some limitations, such as reoperation, or depending on ureteral length. Tissue engineering techniques would provide novel approaches to avoid the limitations. In this study, we fabricated bilayered autologous adipose-derived mesenchymal cell (AMC)-gelatin sheets and transplanted them into rabbits to replace surgically excised ureteral segments. METHODS: AMCs harvested from abdominal adipose tissues of female New Zealand White rabbits were cultured on collagen-coated dishes and labeled with a red fluorescent dye, PKH26, for later identification. Monolayers of AMCs that were cultured on temperature-responsive dishes were harvested by a gelatin hydrogel sheet. Two AMC-gelatin sheets were then overlaid together with the cell sides juxtaposed, forming a bilayered AMC-gelatin sheet. Following each partial ureterectomy of approximately 1 cm, the bilayered autologous AMC-gelatin sheet was transplanted, joining the proximal and distal ends of the remaining the ureter (experimental group; n=9). Control animals were conducted with a bilayered acellular-gelatin sheet (control group; n=9). RESULTS: At 4 and 8 weeks after transplantation, the proximal regions of ureters of control groups exhibited flexures and dilations compared with experimental groups. Both the control and experimental reconstructed ureteral walls had smooth muscle layers; however, those in the experimental reconstructed ureteral walls were significantly thicker and better organized than those in the control groups. In addition, the lumina of the 8-week reconstructed ureteral tissues in experimental groups did not show histological strictures as seen in the control ureters. Some AMCs differentiated into smooth muscle marker-positive cells. The experimental ureteral walls contained smooth muscle cells derived from the PKH26-labeled AMCs and others that were derived through migration and differentiation of cells from the remaining proximal and distal ends of the original ureter. CONCLUSIONS: These results indicated that the bilayered AMC-gelatin sheets could replace and/or reconstruct ureter tissues. Source of Funding: None © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e575 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Noriyuki Ogawa More articles by this author Tetsuya Imamura More articles by this author Tomonori Minagawa More articles by this author Teruyuki Ogawa More articles by this author Osamu Ishizuka More articles by this author Expand All Advertisement PDF downloadLoading ...