MP41-13 DETECTION OF CORE2 β-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE IN POST-DIGITAL RECTAL EXAMINATION URINE IS AN INDEPENDENT PREDICTOR FOR EXTRAPROSTATIC EXTENSION OF PROSTATE CANCER

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research III1 Apr 2014MP41-13 DETECTION OF CORE2 β-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE IN POST-DIGITAL RECTAL EXAMINATION URINE IS AN INDEPENDENT PREDICTOR FOR EXTRAPROSTATIC EXTENSION OF PROSTATE CANCER Yuki Tobisawa, Shingo Hatakeyama, Tohru Yoneyama, Teppei Okamoto, Hayato Yamamoto, Kazuyuki Mori, Akiko Okamoto, Atsushi Imai, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, and Chikara Ohyama Yuki TobisawaYuki Tobisawa More articles by this author , Shingo HatakeyamaShingo Hatakeyama More articles by this author , Tohru YoneyamaTohru Yoneyama More articles by this author , Teppei OkamotoTeppei Okamoto More articles by this author , Hayato YamamotoHayato Yamamoto More articles by this author , Kazuyuki MoriKazuyuki Mori More articles by this author , Akiko OkamotoAkiko Okamoto More articles by this author , Atsushi ImaiAtsushi Imai More articles by this author , Takahiro YoneyamaTakahiro Yoneyama More articles by this author , Yasuhiro HashimotoYasuhiro Hashimoto More articles by this author , Takuya KoieTakuya Koie More articles by this author , and Chikara OhyamaChikara Ohyama More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1230AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives To avoid over treatment of localized prostate cancer (PCa), it is very important to develop a novel biomarker for malignant potential of PCa. Core2 β-1,6-N-acetylglucosaminyltransferase (C2GnT) is an glycosyltransferase which forms core2 branching O-glycan on various glycoproteins. Our research group previously reported that C2GnT expressions were associated with malignant potential of cololectal cancer (Cancer Res, 1997), lung cancer (Cancer Res, 2001), testicular cancer (Int J Cancer, 2010), bladder cancer (EMBO J, 2011) and prostate cancer (Glycobiology, 2005 and AUA 2013). We examined whether detection of C2GnT in post-digital rectal examination (DRE) urine can predict extraprostatic extension. Methods To determine the correlation of C2GnT expression with pathological parameters and biochemical outcome after radical prostatectomy, post-DRE urine specimens of 111 patients with PCa were immunoblotting-based detection of C2GnT expression using the anti-C2GnT monoclonal antibody. Results C2GnT-positive or -negative PCa had not significantly difference of pre-operative parameters (biopsy Gleason score, clinical stage and more). In post-operative parameters of 47 patients of 111 post-DRE urine specimens, C2GnT positive rate in pT2 (22.7%) was significantly lower than that in pT3/pT4 (60.0%) (chi-square-test, p=0.0099). Multivariate analysis revealed Risk stratification (D'Amico) and C2GnT expressions were independent predictor for extra prostatic extension of PCa. Conclusions These results suggest that immunoblotting-based detection of C2GnT in post-DRE urine specimen is an independent and strong predictor of extra prostatic extension before radical prostatectomy. Further clinical trial is necessary to determine the implication of C2GnT as a novel biomarker of prostate cancer. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e455 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yuki Tobisawa More articles by this author Shingo Hatakeyama More articles by this author Tohru Yoneyama More articles by this author Teppei Okamoto More articles by this author Hayato Yamamoto More articles by this author Kazuyuki Mori More articles by this author Akiko Okamoto More articles by this author Atsushi Imai More articles by this author Takahiro Yoneyama More articles by this author Yasuhiro Hashimoto More articles by this author Takuya Koie More articles by this author Chikara Ohyama More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...