MP4-05 CAN NITRIC OXIDE COATING MODIFY POLYPROPILENE MESH IMMUNE-INFLAMMATORY REACTION AND COLLAGEN METABOLISM? IMMUNOHISTOCHEMICAL ANALYSIS IN A RAT MODEL

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research II1 Apr 2014MP4-05 CAN NITRIC OXIDE COATING MODIFY POLYPROPILENE MESH IMMUNE-INFLAMMATORY REACTION AND COLLAGEN METABOLISM? IMMUNOHISTOCHEMICAL ANALYSIS IN A RAT MODEL Alessandro Prudente, Fernando Goulart, Fernandes Dias, Marcelo Ganzarolli Oliveira, and Cassio Luis Zanettini Riccetto Alessandro PrudenteAlessandro Prudente More articles by this author , Fernando GoulartFernando Goulart More articles by this author , Fernandes DiasFernandes Dias More articles by this author , Marcelo Ganzarolli OliveiraMarcelo Ganzarolli Oliveira More articles by this author , and Cassio Luis Zanettini RiccettoCassio Luis Zanettini Riccetto More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.205AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Although the good results achieved with synthetic slings, complications related to vaginal prolapse treatment with polypropylene meshes (PP) cannot be neglected and lead to regulatory agencies warnings around the world. Experimental studies have proven that nitric oxide (NO) is effective to accelerate wound healing, increase angiogenesis and modulate collagen deposition. In a previous study, it was observed that NO coating decreases fibroblastic reaction without change the acute inflammatory response. The aim of this study is to verify the effect of PP coated with NO donor (S-nitrosoglutatione – GSNO) carried by poly (vinyl alcohol) (PVA) in the immunological response, collagen metabolism and angiogenesis in a rat model. METHODS Thirty female rats were submitted to subcutaneous implant of five PP fragments following the protocol: sham (without mesh); uncoated PP (control); PP coated only with PVA; or PP coated with GSNO+PVA in three concentrations: 1, 10 and 70 mMol. Gama irradiation was used to sterilize meshes. Animals were divided into 2 groups (15 each) and were euthanized at 4 days (group 1) and 30 days (group 2). Abdominal wall was excised en bloc for microscopic analyses. The immunohistochemical analysis was done in order to assess: (a) immunologic response (Interleukin 1- IL-1); (b) collagen metabolism (metalloproteinases 2 MMP-2); (c) angiogenesis (surface antigen CD-31). Objective analysis of immunoreactive expression (percent reactive area, total reaction tissue area and vessels concentration) was performed with AxioVision Microscope Software (Karl Zeiss-Germany). RESULTS It was observed higher vessel density in the PVA and 1mM GSNO+PVA group (p=0,0025 e p= 0,0081, respectively). There were higher IL-1 average density in all groups in relation to sham at 4 and 30 days (p=0,0056 and p= 0,0109, respectively). There were no difference among treatments along the time. The total reaction tissue area is higher at 30 than at 4 days for all groups with mesh (p< 0,0001) and higher than the sham in any time of euthansia (p< 0,0001 for 4 days and 30 days). CONCLUSIONS For this experimental model, in proper concentrations, GSNO coating can increase angiogenesis and change immunologic response to mesh implant There was a increase of IL1 expression, without signicant changes in collagen metabolism. The total reaction tissue increases after mesh implant and over time. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e43-e44 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Alessandro Prudente More articles by this author Fernando Goulart More articles by this author Fernandes Dias More articles by this author Marcelo Ganzarolli Oliveira More articles by this author Cassio Luis Zanettini Riccetto More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...