MP4-16 CAN HIGHLY PURIFIED COLLAGEN COATING MODULATE POLYPROPILENE MESH IMMUNE-INFLAMMATORY AND FIBROBLASTIC REACTIONS? IMMUNOHISTOCHEMICAL ANALYSIS IN RAT MODEL

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research II1 Apr 2014MP4-16 CAN HIGHLY PURIFIED COLLAGEN COATING MODULATE POLYPROPILENE MESH IMMUNE-INFLAMMATORY AND FIBROBLASTIC REACTIONS? IMMUNOHISTOCHEMICAL ANALYSIS IN RAT MODEL Fernando Dias, Alessandro Dos Santos, Rodrigo Siniscalchi, Benedicto Vidal, and Cassio Riccetto Fernando DiasFernando Dias More articles by this author , Alessandro Dos SantosAlessandro Dos Santos More articles by this author , Rodrigo SiniscalchiRodrigo Siniscalchi More articles by this author , Benedicto VidalBenedicto Vidal More articles by this author , and Cassio RiccettoCassio Riccetto More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.216AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The use of monofilament polypropylene (PP) mesh as slings has become the standard treatment of urinary incontinence with low risk of extrusion, erosion or vaginal exposure. However, the complications related to vaginal prolapse treatment with PP meshes kits cannot be neglected and had led to regulatory agencies warnings around the World. Collagen is an important immune and inflammatory modulator and has been proposed as a useful biomaterial to increase healing process efficiency. Previous attempts to combine meshes and collagen were based on covering the mesh with a nonporous collagen membrane, which resulted in mesh encapsulation and failure. The aim of this study is to verify the effect of high-purified collagen gel coating in the immune-inflammatory response, host collagen metabolism and angiogenesis around PP mesh. METHODS In twenty female Wistar rats were randomly implanted, at one side of abdominal wall, a 20x10 mm monofilament polypropylene mesh (PP), and, on the other side, the same mesh, but coated with a new highly purified collagen gel prepared at our institution (PPC). Gama irradiation was used to sterilize both meshes preparations. The animals were divided into four sub-groups and were euthanized at 7, 14, 21 and 90 days after implantation. Immunohistochemical analysis was done using specific antibodies: interleukin 1 (IL-1), metalloproteinases 2 and 3 (MMP-2, MMP-3), surface antigen CD-31 and tumor necrosis factor (TNF-α). Objective analysis (percent reactive area, average density and vessels concentration) was performed with AxioVision Microscope Software (Karl Zeiss-Germany). RESULTS Comparative analysis among the four periods and between the 2 groups showed: higher vessel density in the PPC group after 14 days (p=0.002) with significant reduction after 90 days (p=0.002) in this group; decrease of MMP-2 average density in PPC group after 21 and 90 days (p=0.046); more stability in MMP-3’s behavior in PPC group among periods, with MMP-3 percent reactive area showing significant decrease just in PP group after 14 and 90 days (p=0.017) and also for MMP-3 average density, in which reduction was significant after 21 days in PP group, but just after 90 days in PPC (p<0.001). CONCLUSIONS Highly purified collagen coating causes significant changes in angiogenesis and in metalloproteinase's (2 and 3) immune reaction around PP meshes implants in rats. These findings can be useful for improving PP mesh biocompatibility for pelvic floor surgery if such effects could be properly controlled. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e49 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Fernando Dias More articles by this author Alessandro Dos Santos More articles by this author Rodrigo Siniscalchi More articles by this author Benedicto Vidal More articles by this author Cassio Riccetto More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...