MP39-16 ATTRACTIVE STRATEGY FOR TREATMENT OF ADVANCED RENAL CELL CARCINOMA BASED ON VERSICAN EXPRESSION

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research I1 Apr 2015MP39-16 ATTRACTIVE STRATEGY FOR TREATMENT OF ADVANCED RENAL CELL CARCINOMA BASED ON VERSICAN EXPRESSION Yozo Mitsui, Inik Chang, Naoko Arichi, Miho Hiraki, Hiroaki Yasumoto, Shinichiro Fukuhara, Hiroshi Hirata, Soichiro Yamamura, Varahram Shahryari, Guoren Deng, Ruzhu Lan, Sharanjot Saini, Shahana Majid, Hiroaki Shiina, Rajvir Dahiya, and Tanaka Yuichirio Yozo MitsuiYozo Mitsui More articles by this author , Inik ChangInik Chang More articles by this author , Naoko ArichiNaoko Arichi More articles by this author , Miho HirakiMiho Hiraki More articles by this author , Hiroaki YasumotoHiroaki Yasumoto More articles by this author , Shinichiro FukuharaShinichiro Fukuhara More articles by this author , Hiroshi HirataHiroshi Hirata More articles by this author , Soichiro YamamuraSoichiro Yamamura More articles by this author , Varahram ShahryariVarahram Shahryari More articles by this author , Guoren DengGuoren Deng More articles by this author , Ruzhu LanRuzhu Lan More articles by this author , Sharanjot SainiSharanjot Saini More articles by this author , Shahana MajidShahana Majid More articles by this author , Hiroaki ShiinaHiroaki Shiina More articles by this author , Rajvir DahiyaRajvir Dahiya More articles by this author , and Tanaka YuichirioTanaka Yuichirio More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.766AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Management of advanced renal cell carcinoma (RCC) remains a difficult challenge, because of limited therapeutic options and poor patient prognosis. Better understanding of the molecular mechanisms driving RCC progression may contribute to development of more effective therapeutic and diagnostic intervention strategies. Versican, a large extracellular matrix proteoglycan, is thought to generate a hospitable microenvironment for tumor invasion and metastasis in breast and prostate tissues, as well as many other cancer types. However, information regarding versican in RCC is limited. In this study, we investigated the association of versican expression with clinical parameters in RCC cases. METHODS The expression of versican was analyzed using 3 renal tumor cell lines (Caki-2, ACHN, 786-O), and 84 matched clear cell RCC and normal renal tissues. In addition to performing immunoblotting, and reverse transcription and quantitative PCR assays, we also examined whether down-regulation of versican had an effect on the viability, or migratory and invasion properties of RCC cells. All study protocols were approved by the ethics committee of Shimane University Faculty of Medicine in accordance with the 1975 Declaration of Helsinki. RESULTS The mRNA expression of versican in the RCC cell lines was significantly higher than in the nonmalignant HK-2 cell line and also significantly higher in RCC samples as compared to normal kidney samples (p<0.0001). The higher expression of versican was significantly correlated with high stage disease and systematic metastasis (p<0.05). When the expression level of the versican mRNA transcript was divided into 2 groups based on median values, higher expression was significantly associated with overall survival (OS) after a radical nephrectomy (p=0.0235). After transfection of versican siRNA, cell viability, migration, and invasion ability were significantly inhibited in the RCC cell lines. CONCLUSIONS We concluded that versican may be an attractive target for designing improved diagnostic and therapeutic strategies for treatment of advanced RCC. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e460-e461 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yozo Mitsui More articles by this author Inik Chang More articles by this author Naoko Arichi More articles by this author Miho Hiraki More articles by this author Hiroaki Yasumoto More articles by this author Shinichiro Fukuhara More articles by this author Hiroshi Hirata More articles by this author Soichiro Yamamura More articles by this author Varahram Shahryari More articles by this author Guoren Deng More articles by this author Ruzhu Lan More articles by this author Sharanjot Saini More articles by this author Shahana Majid More articles by this author Hiroaki Shiina More articles by this author Rajvir Dahiya More articles by this author Tanaka Yuichirio More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...