MP39-04 N- AND O-GLYCOME ANALYSIS OF SERUM AND URINE FROM BLADDER CANCER PATIENTS USING A HIGH–THROUGHPUT GLYCOBLOTTING METHOD

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research IV1 Apr 2014MP39-04 N- AND O-GLYCOME ANALYSIS OF SERUM AND URINE FROM BLADDER CANCER PATIENTS USING A HIGH–THROUGHPUT GLYCOBLOTTING METHOD Motoi Takeuchi, Hiroshi Kitamura, Taiji Tsukamoto, Naoya Masumori, Maho Amano, Kazuko Hirose, Tetsu Ohashi, and Shin-Ichiro Nishimura Motoi TakeuchiMotoi Takeuchi More articles by this author , Hiroshi KitamuraHiroshi Kitamura More articles by this author , Taiji TsukamotoTaiji Tsukamoto More articles by this author , Naoya MasumoriNaoya Masumori More articles by this author , Maho AmanoMaho Amano More articles by this author , Kazuko HiroseKazuko Hirose More articles by this author , Tetsu OhashiTetsu Ohashi More articles by this author , and Shin-Ichiro NishimuraShin-Ichiro Nishimura More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1319AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Our recent studies of clinical glycomics have indicated that glycoblotting makes possible rapid, large-scale glycome analysis of human sera with high reliability that can be employed to search for novel carbohydrate-related biomarkers in various diseases. However, glycome analysis for bladder cancer (BC) has not been well investigated. Therefore, we evaluated serum and urine glycans in BC patients by utilizing a recently established glycoblotting method to develop novel diagnostic biomarkers for BC. METHODS N- and O-glycan levels in whole serum from 13 male patients diagnosed with muscle-invasive BC were analyzed. As a control, 10 patients with benign prostate hyperplasia (BPH) were also studied and the results were compared. Furthermore, urine N- and O-glycome from 8 patients with muscle-invasive BC and 11 with BPH were analyzed. RESULTS The expression level of 5 glycans significantly increased in BC and 4 of them had tetra-antennary and highly sialylated structure. The one N-glycan whose level decreased had a bisected structure. The levels of three O-glycans were significantly higher in BC than in the control. In examination of urine samples, 16 N-glycans were significantly elevated in BC as compared to the control. Although 11 O-glycans were detected in urine samples, there was no significant difference in the expression levels. CONCLUSIONS The levels of highly branched, sialylated N-glycans and early sialylated O-glycans were increased in sera of BC patients. Moreover, we found that N-glycans increased in urine more than in serum of BC patients. These results suggest that the glycome change in urine directly results from glycoproteins that exist in BC cells. Thus, further large-scale glycan profiling will provide novel biomarkers for diagnosing BC in the near future. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e427 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Motoi Takeuchi More articles by this author Hiroshi Kitamura More articles by this author Taiji Tsukamoto More articles by this author Naoya Masumori More articles by this author Maho Amano More articles by this author Kazuko Hirose More articles by this author Tetsu Ohashi More articles by this author Shin-Ichiro Nishimura More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...