MP36-15 MICRORNA-218 INHIBITS BLADDER CANCER CELL PROLIFERATION, MIGRATION AND INVASION BY TARGETING BMI-1

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research I1 Apr 2015MP36-15 MICRORNA-218 INHIBITS BLADDER CANCER CELL PROLIFERATION, MIGRATION AND INVASION BY TARGETING BMI-1 Pengchao Li, Qiang Lu, Jun Tao, and Chao Qin Pengchao LiPengchao Li More articles by this author , Qiang LuQiang Lu More articles by this author , Jun TaoJun Tao More articles by this author , and Chao QinChao Qin More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.743AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES It is increasingly clear that microRNAs (miRNAs) play an important role in tumorigenesis. Growing evidence suggested that miR-218 was a tumor suppressor in many human cancers. However, its underlying role in bladder cance (BCa) remains unclear. The aim of this study was to explore the effect of miR-218 on the proliferation, migration and invasion of BCa cells. METHODS The expression of miR-218 and BMI-1 in tissues and cell lines were detected by qRT-PCR. The expression of BMI-1, PTEN, AKT and pAKT in cell lines were detected by western blot. Cell viability assay, colony formation assay, wound healing assay and transwell assay in vitro and tumour xenograft studies in vivo were performed to investigate the influence of miR-218 on the proliferation, invasion and migration of the BCa cell lines. Luciferase reporter assay was used to demonstrate whether BMI-1 was a direct target of miR-218. RESULTS The miR-218 expression level was frequently downregulated in BCa tissues compared with normal adjacent tissues. The overexpression of miR-218 significantly inhibited cell proliferation, migration and invasion in BCa cell lines. Luciferase reporter assay showed that BMI-1 was a direct target of miR-218. In addition, we found that miR-218 suppressed the expression of BMI-1 and its downstream target (PTEN) and participated in the regulation of phospho-AKT (pAKT). CONCLUSIONS Our findings indicated that miR-218 functions as tumour suppressor in BCa, and the miR-218/BMI-1/PTEN/AKT axis may provide novel diagnostic and therapeutic strategies for the treatment of BCa. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e434-e435 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Pengchao Li More articles by this author Qiang Lu More articles by this author Jun Tao More articles by this author Chao Qin More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...